Interaction of Escherichia coli and its culture supernatant with Vibrio vulnificus during biofilm formation

Han Min Ohn, Tamaki Mizuno, Yuki Sudo, Shin Ichi Miyoshi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Vibrio vulnificus is a foodborne pathogen causing septicemia with high mortality rate. In this study, we explored how Escherichia coli, one of the commensal bacteria in the human gastrointestinal tract, can interact with V. vulnificus. Our study results show that the amount of biofilm produced by V. vulnificus was reduced in the presence of E. coli ATCC 35218, although the growth of V. vulnificus L-180 remained unaffected. We also detected an antibiofilm effect of E. coli culture supernatant against V. vulnificus, which could not be reduced even after heat treatment. These findings indicate that E. coli and its culture supernatant may be suitable to prevent biofilm formation by V. vulnificus. By contrast, live cells of V. vulnificus could reduce the amount of preformed E. coli biofilm, but its culture supernatant could not. This suggests that the cell-associated factors contribute toward reduction in E. coli biofilm. Therefore, we speculate that ingestion of an infectious dose of V. vulnificus might induce dislodging of the commensal bacteria from the intestinal epithelia and thus can colonize to initiate the infection.

Original languageEnglish
Pages (from-to)593-601
Number of pages9
JournalMICROBIOLOGY and IMMUNOLOGY
Volume64
Issue number9
DOIs
Publication statusPublished - Sep 1 2020

Keywords

  • Escherichia coli
  • Vibrio vulnificus
  • antibiofilm effect
  • biofilm
  • culture supernatant

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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