Interaction of AP-1 and the ctgf gene: A possible driver of chondrocyte hypertrophy in growth cartilage

Norifumi Moritani, Satoshi Kubota, Takanori Eguchi, Tomohiro Fukunaga, Takashi Yamashiro, Teruko Takano-Yamamoto, Hideki Tahara, Kazumi Ohyama, Toshio Sugahara, Masaharu Takigawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The expression of the connective tissue growth factor (ctgf) gene increases along with the differentiation of growth cartilage cells, and the highest expression is observed in the hypertrophic stage. Similarly, recent reports demonstrated c-fos expression in chondrocytes in the early hypertrophic zone of growth cartilage, and suggested that the c-fos gene may play a crucial role in the regulation of hypertrophic differentiation. A chondrocytic human cell line, HCS-2/8, is known to retain a variety of chondrocytic phenotypes. When such cells were kept overconfluent, they expressed increasing levels of c-fos transcripts along a time course phenotypically similar to that of hypertrophic differentiation. Moreover, by using a competitive electromobility-shift assay, we found that AP-1, a Fos/Jun heterodimer, in HCS-2/8 was capable of binding not only to a typical AP-1-binding DNA fragment but also to the enhancer fragment of the ctgf gene. Based on the findings above, we hypothesize that, prior to hypertrophic differentiation, AP-1-related oncogenes are activated and that their gene products subsequently activate ctgf gene expression, which might eventually induce hypertrophy.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalJournal of Bone and Mineral Metabolism
Volume21
Issue number4
Publication statusPublished - 2003

Fingerprint

Connective Tissue Growth Factor
Transcription Factor AP-1
Chondrocytes
Hypertrophy
Cartilage
Growth
Genes
fos Genes
Oncogenes
Phenotype
Gene Expression
Cell Line
DNA

Keywords

  • AP-1
  • c-fos
  • Cartilage
  • CTGF

ASJC Scopus subject areas

  • Endocrinology

Cite this

Interaction of AP-1 and the ctgf gene : A possible driver of chondrocyte hypertrophy in growth cartilage. / Moritani, Norifumi; Kubota, Satoshi; Eguchi, Takanori; Fukunaga, Tomohiro; Yamashiro, Takashi; Takano-Yamamoto, Teruko; Tahara, Hideki; Ohyama, Kazumi; Sugahara, Toshio; Takigawa, Masaharu.

In: Journal of Bone and Mineral Metabolism, Vol. 21, No. 4, 2003, p. 205-210.

Research output: Contribution to journalArticle

Moritani, Norifumi ; Kubota, Satoshi ; Eguchi, Takanori ; Fukunaga, Tomohiro ; Yamashiro, Takashi ; Takano-Yamamoto, Teruko ; Tahara, Hideki ; Ohyama, Kazumi ; Sugahara, Toshio ; Takigawa, Masaharu. / Interaction of AP-1 and the ctgf gene : A possible driver of chondrocyte hypertrophy in growth cartilage. In: Journal of Bone and Mineral Metabolism. 2003 ; Vol. 21, No. 4. pp. 205-210.
@article{f0f965c7e4cc4e51997ae82f7f8edc28,
title = "Interaction of AP-1 and the ctgf gene: A possible driver of chondrocyte hypertrophy in growth cartilage",
abstract = "The expression of the connective tissue growth factor (ctgf) gene increases along with the differentiation of growth cartilage cells, and the highest expression is observed in the hypertrophic stage. Similarly, recent reports demonstrated c-fos expression in chondrocytes in the early hypertrophic zone of growth cartilage, and suggested that the c-fos gene may play a crucial role in the regulation of hypertrophic differentiation. A chondrocytic human cell line, HCS-2/8, is known to retain a variety of chondrocytic phenotypes. When such cells were kept overconfluent, they expressed increasing levels of c-fos transcripts along a time course phenotypically similar to that of hypertrophic differentiation. Moreover, by using a competitive electromobility-shift assay, we found that AP-1, a Fos/Jun heterodimer, in HCS-2/8 was capable of binding not only to a typical AP-1-binding DNA fragment but also to the enhancer fragment of the ctgf gene. Based on the findings above, we hypothesize that, prior to hypertrophic differentiation, AP-1-related oncogenes are activated and that their gene products subsequently activate ctgf gene expression, which might eventually induce hypertrophy.",
keywords = "AP-1, c-fos, Cartilage, CTGF",
author = "Norifumi Moritani and Satoshi Kubota and Takanori Eguchi and Tomohiro Fukunaga and Takashi Yamashiro and Teruko Takano-Yamamoto and Hideki Tahara and Kazumi Ohyama and Toshio Sugahara and Masaharu Takigawa",
year = "2003",
language = "English",
volume = "21",
pages = "205--210",
journal = "Journal of Bone and Mineral Metabolism",
issn = "0914-8779",
publisher = "Springer Japan",
number = "4",

}

TY - JOUR

T1 - Interaction of AP-1 and the ctgf gene

T2 - A possible driver of chondrocyte hypertrophy in growth cartilage

AU - Moritani, Norifumi

AU - Kubota, Satoshi

AU - Eguchi, Takanori

AU - Fukunaga, Tomohiro

AU - Yamashiro, Takashi

AU - Takano-Yamamoto, Teruko

AU - Tahara, Hideki

AU - Ohyama, Kazumi

AU - Sugahara, Toshio

AU - Takigawa, Masaharu

PY - 2003

Y1 - 2003

N2 - The expression of the connective tissue growth factor (ctgf) gene increases along with the differentiation of growth cartilage cells, and the highest expression is observed in the hypertrophic stage. Similarly, recent reports demonstrated c-fos expression in chondrocytes in the early hypertrophic zone of growth cartilage, and suggested that the c-fos gene may play a crucial role in the regulation of hypertrophic differentiation. A chondrocytic human cell line, HCS-2/8, is known to retain a variety of chondrocytic phenotypes. When such cells were kept overconfluent, they expressed increasing levels of c-fos transcripts along a time course phenotypically similar to that of hypertrophic differentiation. Moreover, by using a competitive electromobility-shift assay, we found that AP-1, a Fos/Jun heterodimer, in HCS-2/8 was capable of binding not only to a typical AP-1-binding DNA fragment but also to the enhancer fragment of the ctgf gene. Based on the findings above, we hypothesize that, prior to hypertrophic differentiation, AP-1-related oncogenes are activated and that their gene products subsequently activate ctgf gene expression, which might eventually induce hypertrophy.

AB - The expression of the connective tissue growth factor (ctgf) gene increases along with the differentiation of growth cartilage cells, and the highest expression is observed in the hypertrophic stage. Similarly, recent reports demonstrated c-fos expression in chondrocytes in the early hypertrophic zone of growth cartilage, and suggested that the c-fos gene may play a crucial role in the regulation of hypertrophic differentiation. A chondrocytic human cell line, HCS-2/8, is known to retain a variety of chondrocytic phenotypes. When such cells were kept overconfluent, they expressed increasing levels of c-fos transcripts along a time course phenotypically similar to that of hypertrophic differentiation. Moreover, by using a competitive electromobility-shift assay, we found that AP-1, a Fos/Jun heterodimer, in HCS-2/8 was capable of binding not only to a typical AP-1-binding DNA fragment but also to the enhancer fragment of the ctgf gene. Based on the findings above, we hypothesize that, prior to hypertrophic differentiation, AP-1-related oncogenes are activated and that their gene products subsequently activate ctgf gene expression, which might eventually induce hypertrophy.

KW - AP-1

KW - c-fos

KW - Cartilage

KW - CTGF

UR - http://www.scopus.com/inward/record.url?scp=1942518942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1942518942&partnerID=8YFLogxK

M3 - Article

C2 - 12811624

AN - SCOPUS:1942518942

VL - 21

SP - 205

EP - 210

JO - Journal of Bone and Mineral Metabolism

JF - Journal of Bone and Mineral Metabolism

SN - 0914-8779

IS - 4

ER -