Interaction between two auxin-resistant mutants and their effects on lateral root formation in rice (Oryza sativa L.)

Tory Chhun, Shin Taketa, Seiji Tsurumi, Masahiko Ichii

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Since root elongation is very sensitive to auxin, screening for reduced inhibition in root elongation has been an important method for the detection of auxin-resistant mutants. Two recessive auxin-resistant lines of rice (Oryza sativa L. ssp. indica cv. IR8), arm1 and arm2, have been isolated by screening for resistance to 2,4-dichlorophenoxyacetic acid (2,4-D), arm1 displays a variety of morphological defects including reduced lateral root formation, increased seminal root elongation, reduced root diameter, and impaired xylem development in roots, while the arm2 phenotype is almost similar to wild-type IR8 except for a slightly reduced lateral root formation, impaired xylem development in roots and an enhanced plant height. Although the growth of arm2 roots exhibited a resistance to 2,4-D, it was sensitive to 1-naphthaleneacetic acid (NAA) as the wild type. At the same time, the arm2 roots showed a reduced [14C]2,4-D uptake while uptake of [3H]NAA was normal, suggesting that the resistance to 2,4-D of arm2 roots is due to a defect in 2,4-D uptake. To investigate the possible interaction between arm1 and arm2 genes, a double mutant has been constructed. The roots of arm1 arm2 double mutant were more resistant to 2,4-D and formed fewer lateral roots than those of either single mutant, suggesting that the two genes show synergistic effects with respect to both auxin response and lateral root formation. By contrast, all these mutants displayed the normal gravitropic response in roots, as did the wild-type plants. Taken together, Arm1 and Arm2 genes seem to function in different processes in the auxin-response pathways leading to lateral root formation.

Original languageEnglish
Pages (from-to)2701-2708
Number of pages8
JournalJournal of Experimental Botany
Volume54
Issue number393
DOIs
Publication statusPublished - Dec 2003
Externally publishedYes

Fingerprint

2,4-Dichlorophenoxyacetic Acid
Indoleacetic Acids
auxins
Oryza sativa
rice
mutants
2,4-D
Xylem
root growth
Naphthaleneacetic Acids
Genes
Plant Roots
uptake mechanisms
naphthaleneacetic acid
xylem
Oryza
screening
genes
Phenotype
Growth

Keywords

  • 2,4-dichlorophenoxyacetic acid
  • Auxin influx
  • Auxin-resistant mutant
  • Gravitropic response
  • Lateral root formation
  • Rice root
  • Root elongation
  • Xylem development

ASJC Scopus subject areas

  • Plant Science

Cite this

Interaction between two auxin-resistant mutants and their effects on lateral root formation in rice (Oryza sativa L.). / Chhun, Tory; Taketa, Shin; Tsurumi, Seiji; Ichii, Masahiko.

In: Journal of Experimental Botany, Vol. 54, No. 393, 12.2003, p. 2701-2708.

Research output: Contribution to journalArticle

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abstract = "Since root elongation is very sensitive to auxin, screening for reduced inhibition in root elongation has been an important method for the detection of auxin-resistant mutants. Two recessive auxin-resistant lines of rice (Oryza sativa L. ssp. indica cv. IR8), arm1 and arm2, have been isolated by screening for resistance to 2,4-dichlorophenoxyacetic acid (2,4-D), arm1 displays a variety of morphological defects including reduced lateral root formation, increased seminal root elongation, reduced root diameter, and impaired xylem development in roots, while the arm2 phenotype is almost similar to wild-type IR8 except for a slightly reduced lateral root formation, impaired xylem development in roots and an enhanced plant height. Although the growth of arm2 roots exhibited a resistance to 2,4-D, it was sensitive to 1-naphthaleneacetic acid (NAA) as the wild type. At the same time, the arm2 roots showed a reduced [14C]2,4-D uptake while uptake of [3H]NAA was normal, suggesting that the resistance to 2,4-D of arm2 roots is due to a defect in 2,4-D uptake. To investigate the possible interaction between arm1 and arm2 genes, a double mutant has been constructed. The roots of arm1 arm2 double mutant were more resistant to 2,4-D and formed fewer lateral roots than those of either single mutant, suggesting that the two genes show synergistic effects with respect to both auxin response and lateral root formation. By contrast, all these mutants displayed the normal gravitropic response in roots, as did the wild-type plants. Taken together, Arm1 and Arm2 genes seem to function in different processes in the auxin-response pathways leading to lateral root formation.",
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KW - Xylem development

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