Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site

Pier Paolo D'Avino, Tetsuya Takeda, Luisa Capalbo, Wei Zhang, Kathryn S. Lilley, Ernest D. Laue, David M. Glover

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Anillin, one of the first factors recruited to the cleavage site during cytokinesis, interacts with actin, myosin II and septins, and is essential for proper organization of the actomyosin contractile ring. We employed affinity-purification methodology coupled with mass spectrometry to identify Anillin-interacting molecules in Drosophila cells. We isolated several actin and myosin proteins, three of the five Drosophila septins and RacGAP50C (Tum), a component of the centralspindlin complex. Using drug and RNA interference (RNAO treatments we established that F-actin is essential for Anillin cortical localization in prometaphase but not for its accumulation at the cleavage furrow after anaphase onset. Moreover, septins were not recruited to the cleavage site in cells in which Anillin was knocked down by RNAi, but localized to central-spindle microtubules, suggesting that septins travel along microtubules to interact with Anillin at the furrow. Finally, we demonstrate that RacGAP50C is necessary for Anillin accumulation at the furrow and that the two proteins colocalize in vivo and interact in vitro. Thus, in addition to its role in activating RhoA signalling, RacGAP50C also controls, the proper assembly of the actomyosin ring by interacting with Anillin at the cleavage furrow.

Original languageEnglish
Pages (from-to)1151-1158
Number of pages8
JournalJournal of Cell Science
Volume121
Issue number8
DOIs
Publication statusPublished - Apr 15 2008
Externally publishedYes

Fingerprint

Actomyosin
Microtubules
Cell Division
Septins
Actins
RNA Interference
Drosophila
Prometaphase
Myosin Type II
Anaphase
Cytokinesis
Myosins
anillin
Mass Spectrometry
Proteins
Pharmaceutical Preparations

Keywords

  • Affinity purification
  • Anillin
  • Centralspindlin
  • Protein complex

ASJC Scopus subject areas

  • Cell Biology

Cite this

Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. / D'Avino, Pier Paolo; Takeda, Tetsuya; Capalbo, Luisa; Zhang, Wei; Lilley, Kathryn S.; Laue, Ernest D.; Glover, David M.

In: Journal of Cell Science, Vol. 121, No. 8, 15.04.2008, p. 1151-1158.

Research output: Contribution to journalArticle

D'Avino, Pier Paolo ; Takeda, Tetsuya ; Capalbo, Luisa ; Zhang, Wei ; Lilley, Kathryn S. ; Laue, Ernest D. ; Glover, David M. / Interaction between Anillin and RacGAP50C connects the actomyosin contractile ring with spindle microtubules at the cell division site. In: Journal of Cell Science. 2008 ; Vol. 121, No. 8. pp. 1151-1158.
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