Abstract
Electron microscopy of ultrathin sections stained with cationic iron colloid revealed that the rat pineal gland is provided with wide and intensely negative-charged pericapillary spaces. Light microscopically, the negative charging of the pericapillary spaces was completely eliminated by digestion with hyaluronidase and chondroitinase ABC. This pericapillary negative charging was also erased by digestion with collagenase. The results indicate that the negative charging is derived from sulfated proteoglycans which are bound to collagen molecules. These sulfated proteoglycans in the pericapillary spaces may retain numerous water molecules to form a tissue gel, and so act as a selective sieve regulating the passage of tissue molecules.
| Original language | English |
|---|---|
| Pages (from-to) | 485-494 |
| Number of pages | 10 |
| Journal | Archives of Histology and Cytology |
| Volume | 63 |
| Issue number | 5 |
| Publication status | Published - 2000 |
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ASJC Scopus subject areas
- Anatomy
- Histology
Cite this
Intensely negative-charged pericapillary spaces in the rat pineal gland. / Taguchi, Takehito; Kosaka, M.; Murakami, S.; Ohtsuka, Aiji; Murakami, T.
In: Archives of Histology and Cytology, Vol. 63, No. 5, 2000, p. 485-494.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Intensely negative-charged pericapillary spaces in the rat pineal gland
AU - Taguchi, Takehito
AU - Kosaka, M.
AU - Murakami, S.
AU - Ohtsuka, Aiji
AU - Murakami, T.
PY - 2000
Y1 - 2000
N2 - Electron microscopy of ultrathin sections stained with cationic iron colloid revealed that the rat pineal gland is provided with wide and intensely negative-charged pericapillary spaces. Light microscopically, the negative charging of the pericapillary spaces was completely eliminated by digestion with hyaluronidase and chondroitinase ABC. This pericapillary negative charging was also erased by digestion with collagenase. The results indicate that the negative charging is derived from sulfated proteoglycans which are bound to collagen molecules. These sulfated proteoglycans in the pericapillary spaces may retain numerous water molecules to form a tissue gel, and so act as a selective sieve regulating the passage of tissue molecules.
AB - Electron microscopy of ultrathin sections stained with cationic iron colloid revealed that the rat pineal gland is provided with wide and intensely negative-charged pericapillary spaces. Light microscopically, the negative charging of the pericapillary spaces was completely eliminated by digestion with hyaluronidase and chondroitinase ABC. This pericapillary negative charging was also erased by digestion with collagenase. The results indicate that the negative charging is derived from sulfated proteoglycans which are bound to collagen molecules. These sulfated proteoglycans in the pericapillary spaces may retain numerous water molecules to form a tissue gel, and so act as a selective sieve regulating the passage of tissue molecules.
UR - http://www.scopus.com/inward/record.url?scp=0034491847&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034491847&partnerID=8YFLogxK
M3 - Article
C2 - 11201207
AN - SCOPUS:0034491847
VL - 63
SP - 485
EP - 494
JO - Archives of Histology and Cytology
JF - Archives of Histology and Cytology
SN - 0914-9465
IS - 5
ER -