Intensely negative-charged pericapillary spaces in the rat pineal gland

Takehito Taguchi, M. Kosaka, S. Murakami, Aiji Ohtsuka, T. Murakami

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Electron microscopy of ultrathin sections stained with cationic iron colloid revealed that the rat pineal gland is provided with wide and intensely negative-charged pericapillary spaces. Light microscopically, the negative charging of the pericapillary spaces was completely eliminated by digestion with hyaluronidase and chondroitinase ABC. This pericapillary negative charging was also erased by digestion with collagenase. The results indicate that the negative charging is derived from sulfated proteoglycans which are bound to collagen molecules. These sulfated proteoglycans in the pericapillary spaces may retain numerous water molecules to form a tissue gel, and so act as a selective sieve regulating the passage of tissue molecules.

Original languageEnglish
Pages (from-to)485-494
Number of pages10
JournalArchives of Histology and Cytology
Volume63
Issue number5
Publication statusPublished - 2000

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Pineal Gland
Proteoglycans
Digestion
Chondroitin ABC Lyase
Hyaluronoglucosaminidase
Colloids
Collagenases
Electron Microscopy
Collagen
Iron
Gels
Light
Water

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Intensely negative-charged pericapillary spaces in the rat pineal gland. / Taguchi, Takehito; Kosaka, M.; Murakami, S.; Ohtsuka, Aiji; Murakami, T.

In: Archives of Histology and Cytology, Vol. 63, No. 5, 2000, p. 485-494.

Research output: Contribution to journalArticle

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