Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells

E. Tsuruga, A. Sato, T. Ueki, K. Nakashima, Y. Nakatomi, H. Ishikawa, T. Yajima, Yoshihiko Sawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalTissue and Cell
Volume41
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Microfibrils
Periodontal Ligament
Integrins
Fibroblasts
Fibrillin-1
Fluorescent Antibody Technique
Homeostasis
Western Blotting

Keywords

  • Fibrillin
  • Integrin
  • Microfibril
  • Oxytalan fiber
  • Periodontal ligament

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells. / Tsuruga, E.; Sato, A.; Ueki, T.; Nakashima, K.; Nakatomi, Y.; Ishikawa, H.; Yajima, T.; Sawa, Yoshihiko.

In: Tissue and Cell, Vol. 41, No. 2, 2009, p. 85-89.

Research output: Contribution to journalArticle

Tsuruga, E, Sato, A, Ueki, T, Nakashima, K, Nakatomi, Y, Ishikawa, H, Yajima, T & Sawa, Y 2009, 'Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells', Tissue and Cell, vol. 41, no. 2, pp. 85-89. https://doi.org/10.1016/j.tice.2008.07.005
Tsuruga, E. ; Sato, A. ; Ueki, T. ; Nakashima, K. ; Nakatomi, Y. ; Ishikawa, H. ; Yajima, T. ; Sawa, Yoshihiko. / Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells. In: Tissue and Cell. 2009 ; Vol. 41, No. 2. pp. 85-89.
@article{39bbcdd5e3d5431abbf2502feab02523,
title = "Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells",
abstract = "Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.",
keywords = "Fibrillin, Integrin, Microfibril, Oxytalan fiber, Periodontal ligament",
author = "E. Tsuruga and A. Sato and T. Ueki and K. Nakashima and Y. Nakatomi and H. Ishikawa and T. Yajima and Yoshihiko Sawa",
year = "2009",
doi = "10.1016/j.tice.2008.07.005",
language = "English",
volume = "41",
pages = "85--89",
journal = "Tissue and Cell",
issn = "0040-8166",
publisher = "Churchill Livingstone",
number = "2",

}

TY - JOUR

T1 - Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells

AU - Tsuruga, E.

AU - Sato, A.

AU - Ueki, T.

AU - Nakashima, K.

AU - Nakatomi, Y.

AU - Ishikawa, H.

AU - Yajima, T.

AU - Sawa, Yoshihiko

PY - 2009

Y1 - 2009

N2 - Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.

AB - Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.

KW - Fibrillin

KW - Integrin

KW - Microfibril

KW - Oxytalan fiber

KW - Periodontal ligament

UR - http://www.scopus.com/inward/record.url?scp=60749125001&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60749125001&partnerID=8YFLogxK

U2 - 10.1016/j.tice.2008.07.005

DO - 10.1016/j.tice.2008.07.005

M3 - Article

C2 - 18789468

AN - SCOPUS:60749125001

VL - 41

SP - 85

EP - 89

JO - Tissue and Cell

JF - Tissue and Cell

SN - 0040-8166

IS - 2

ER -