Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells

E. Tsuruga, A. Sato, T. Ueki, K. Nakashima, Y. Nakatomi, H. Ishikawa, T. Yajima, Y. Sawa

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalTissue and Cell
Volume41
Issue number2
DOIs
Publication statusPublished - Apr 2009
Externally publishedYes

Keywords

  • Fibrillin
  • Integrin
  • Microfibril
  • Oxytalan fiber
  • Periodontal ligament

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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