Abstract
Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.
| Original language | English |
|---|---|
| Pages (from-to) | 85-89 |
| Number of pages | 5 |
| Journal | Tissue and Cell |
| Volume | 41 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2009 |
| Externally published | Yes |
Fingerprint
Keywords
- Fibrillin
- Integrin
- Microfibril
- Oxytalan fiber
- Periodontal ligament
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells. / Tsuruga, E.; Sato, A.; Ueki, T.; Nakashima, K.; Nakatomi, Y.; Ishikawa, H.; Yajima, T.; Sawa, Yoshihiko.
In: Tissue and Cell, Vol. 41, No. 2, 2009, p. 85-89.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells
AU - Tsuruga, E.
AU - Sato, A.
AU - Ueki, T.
AU - Nakashima, K.
AU - Nakatomi, Y.
AU - Ishikawa, H.
AU - Yajima, T.
AU - Sawa, Yoshihiko
PY - 2009
Y1 - 2009
N2 - Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.
AB - Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.
KW - Fibrillin
KW - Integrin
KW - Microfibril
KW - Oxytalan fiber
KW - Periodontal ligament
UR - http://www.scopus.com/inward/record.url?scp=60749125001&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=60749125001&partnerID=8YFLogxK
U2 - 10.1016/j.tice.2008.07.005
DO - 10.1016/j.tice.2008.07.005
M3 - Article
C2 - 18789468
AN - SCOPUS:60749125001
VL - 41
SP - 85
EP - 89
JO - Tissue and Cell
JF - Tissue and Cell
SN - 0040-8166
IS - 2
ER -