Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells

E. Tsuruga; A. Sato; T. Ueki; K. Nakashima; Y. Nakatomi; H. Ishikawa; T. Yajima; Y. Sawa

doi:10.1016/j.tice.2008.07.005

Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells

E. Tsuruga, A. Sato, T. Ueki, K. Nakashima, Y. Nakatomi, H. Ishikawa, T. Yajima, Y. Sawa

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.

Original language	English
Pages (from-to)	85-89
Number of pages	5
Journal	Tissue and Cell
Volume	41
Issue number	2
DOIs	https://doi.org/10.1016/j.tice.2008.07.005
Publication status	Published - Apr 2009
Externally published	Yes

Keywords

Fibrillin
Integrin
Microfibril
Oxytalan fiber
Periodontal ligament

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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title = "Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells",

abstract = "Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.",

keywords = "Fibrillin, Integrin, Microfibril, Oxytalan fiber, Periodontal ligament",

author = "E. Tsuruga and A. Sato and T. Ueki and K. Nakashima and Y. Nakatomi and H. Ishikawa and T. Yajima and Y. Sawa",

note = "Funding Information: This work was supported by the Academic Sciences Frontier Project, the Nakatomi Foundation and Grants-in-Aid for Scientific Research (Nos. 17591920, 19592391) from the Ministry of Education, Science, Sports and Culture of Japan.",

year = "2009",

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doi = "10.1016/j.tice.2008.07.005",

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volume = "41",

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AU - Tsuruga, E.

AU - Sato, A.

AU - Ueki, T.

AU - Nakashima, K.

AU - Nakatomi, Y.

AU - Ishikawa, H.

AU - Yajima, T.

AU - Sawa, Y.

N1 - Funding Information: This work was supported by the Academic Sciences Frontier Project, the Nakatomi Foundation and Grants-in-Aid for Scientific Research (Nos. 17591920, 19592391) from the Ministry of Education, Science, Sports and Culture of Japan.

PY - 2009/4

Y1 - 2009/4

N2 - Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.

AB - Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.

KW - Fibrillin

KW - Integrin

KW - Microfibril

KW - Oxytalan fiber

KW - Periodontal ligament

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Integrin αvβ3 regulates microfibril assembly in human periodontal ligament cells

Abstract

Keywords

ASJC Scopus subject areas

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