Abstract
Fibrillin-1 is the major structural component of extracellular microfibrils. However, the mechanism by which extracellular fibrillin-1 assembles into microfibrils is not fully understood. Fibrillin-1 contains the Arg-Gly-Asp (RGD) motif, which may allow binding to RGD-recognizing integrins. We hypothesized that integrin αvβ3 on the cell surface of human periodontal ligament (PDL) fibroblasts may influence fibrillin-1 assembly into cell/matrix layers. We treated PDL fibroblasts with an integrin αvβ3-specific antagonist to examine fibrillin-1 assembly. Western blotting and immunofluorescence analysis showed that treatment with the integrin αvβ3 antagonist at 5 μM clearly abolished fibrillin-1 deposition. These results provide for the first time evidence that integrin αvβ3 regulates extracellular assembly of fibrillin-1, thereby modulating cell-mediated homeostasis of microfibrils.
Original language | English |
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Pages (from-to) | 85-89 |
Number of pages | 5 |
Journal | Tissue and Cell |
Volume | 41 |
Issue number | 2 |
DOIs | |
Publication status | Published - Apr 2009 |
Externally published | Yes |
Keywords
- Fibrillin
- Integrin
- Microfibril
- Oxytalan fiber
- Periodontal ligament
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology