Integration of SCP1, a giant linear plasmid, into the Streptomyces coelicolor chromosome

Haruyasu Kinashi, Miyuki Shimaji-Murayama, Tadashi Hanafusa

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

SCP1, coding for the methylenomycin biosynthetic genes in Streptomyces coelicolor, is a giant linear plasmid of 350 kb. Extensive physical characterization revealed that SCP1 has unusually long terminal inverted repeats (TIR) of about 80 kb on both ends and an insertion sequence, IS466, at the end of the right TIR (TIR-R), and the 5′-ends are attached to a terminal protein. In the NF strain S. coelicolor 2612, SCP1 is integrated into the chromosome at the 9-o'clock position. Analysis of the two junctions between the SCP1 DNA and the chromosomal DNA revealed that the left junction had an almost intact left terminus of SCP1, while the right junction was composed of IS466, completely deleting TIR-R. Based on these results, we presented a possible formation mechanism of the NF strain, which is characterized by integration of SCP1 into the chromosome via an interaction of the target site and the combined ends of the racket-frame structure of SCP1 followed by deletion of TIR-R. We also hypothesized that this type of integration of a giant linear plasmid might be involved in the origin and distribution of the chromosomal antibiotic biosynthetic gene clusters in microorganisms.

Original languageEnglish
Pages (from-to)35-41
Number of pages7
JournalGene
Volume115
Issue number1-2
DOIs
Publication statusPublished - Jun 15 1992

Fingerprint

Streptomyces coelicolor
Terminal Repeat Sequences
Plasmids
Chromosomes
Chromosomes, Human, Pair 9
Insertional Mutagenesis
DNA
Multigene Family
Anti-Bacterial Agents
Genes

Keywords

  • horizontal transfer of antibiotic biosynthesis
  • IS466
  • methylenomycin biosynthesis
  • racket frame model
  • Recombinant DNA
  • terminal protein

ASJC Scopus subject areas

  • Genetics

Cite this

Integration of SCP1, a giant linear plasmid, into the Streptomyces coelicolor chromosome. / Kinashi, Haruyasu; Shimaji-Murayama, Miyuki; Hanafusa, Tadashi.

In: Gene, Vol. 115, No. 1-2, 15.06.1992, p. 35-41.

Research output: Contribution to journalArticle

Kinashi, Haruyasu ; Shimaji-Murayama, Miyuki ; Hanafusa, Tadashi. / Integration of SCP1, a giant linear plasmid, into the Streptomyces coelicolor chromosome. In: Gene. 1992 ; Vol. 115, No. 1-2. pp. 35-41.
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