Integration of danger peptide signals with herbivore-associated molecular pattern signaling amplifies anti-herbivore defense responses in rice

Tomonori Shinya, Shigetaka Yasuda, Kiwamu Hyoudou, Rena Tani, Yuko Hojo, Yuka Fujiwara, Kei Hiruma, Takuma Ishizaki, Yasunari Fujita, Yusuke Saijo, Ivan Galis

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Plant defense against herbivores is modulated by herbivore-associated molecular patterns (HAMPs) from oral secretions (OS) and/or saliva of insects. Furthermore, feeding wounds initiate plant self-damage responses modulated by danger-associated molecular patterns (DAMPs) such as immune defense-promoting plant elicitor peptides (Peps). While temporal and spatial co-existence of both patterns during herbivory implies a possibility of their close interaction, the molecular mechanisms remain undetermined. Here we report that exogenous application of rice (Oryza sativa) peptides (OsPeps) can elicit multiple defense responses in rice cell cultures. Specific activation of OsPROPEP3 gene transcripts in rice leaves by wounding and OS treatments further suggests a possible involvement of the OsPep3 peptide in rice–herbivore interactions. Correspondingly, we found that simultaneous application of OsPep3 and Mythimna loreyi OS significantly amplifies an array of defense responses in rice cells, including mitogen-activated protein kinase activation, and generation of defense-related hormones and metabolites. The induction of OsPROPEP3/4 by OsPep3 points to a positive auto-feedback loop in OsPep signaling which may contribute to additional enhancement of defense signal(s). Finally, the overexpression of the OsPep receptor OsPEPR1 increases the sensitivity of rice plants not only to the cognate OsPeps but also to OS signals. Our findings collectively suggest that HAMP–DAMP signal integration provides a critical step in the amplification of defense signaling in plants.

Original languageEnglish
Pages (from-to)626-637
Number of pages12
JournalPlant Journal
Volume94
Issue number4
DOIs
Publication statusPublished - May 1 2018

Fingerprint

Herbivory
Protein Sorting Signals
signal peptide
herbivores
mouth
rice
secretion
peptides
Mythimna loreyi
Peptides
Oryza sativa
gene activation
saliva
mitogen-activated protein kinase
automobiles
animal injuries
cell culture
hormones
Oryza
Mitogen-Activated Protein Kinases

Keywords

  • danger-associated molecular patterns
  • herbivore-associated molecular patterns
  • Oryza sativa
  • OsPeps
  • plant immunity
  • plant–herbivore interactions
  • rice
  • synergistic effects

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

Cite this

Integration of danger peptide signals with herbivore-associated molecular pattern signaling amplifies anti-herbivore defense responses in rice. / Shinya, Tomonori; Yasuda, Shigetaka; Hyoudou, Kiwamu; Tani, Rena; Hojo, Yuko; Fujiwara, Yuka; Hiruma, Kei; Ishizaki, Takuma; Fujita, Yasunari; Saijo, Yusuke; Galis, Ivan.

In: Plant Journal, Vol. 94, No. 4, 01.05.2018, p. 626-637.

Research output: Contribution to journalArticle

Shinya, Tomonori ; Yasuda, Shigetaka ; Hyoudou, Kiwamu ; Tani, Rena ; Hojo, Yuko ; Fujiwara, Yuka ; Hiruma, Kei ; Ishizaki, Takuma ; Fujita, Yasunari ; Saijo, Yusuke ; Galis, Ivan. / Integration of danger peptide signals with herbivore-associated molecular pattern signaling amplifies anti-herbivore defense responses in rice. In: Plant Journal. 2018 ; Vol. 94, No. 4. pp. 626-637.
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AU - Shinya, Tomonori

AU - Yasuda, Shigetaka

AU - Hyoudou, Kiwamu

AU - Tani, Rena

AU - Hojo, Yuko

AU - Fujiwara, Yuka

AU - Hiruma, Kei

AU - Ishizaki, Takuma

AU - Fujita, Yasunari

AU - Saijo, Yusuke

AU - Galis, Ivan

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