Integrated transcriptomic and proteomic analyses of a molecular mechanism of radular teeth biomineralization in Cryptochiton stelleri

Michiko Nemoto, Dongni Ren, Steven Herrera, Songqin Pan, Takashi Tamura, Kenji Inagaki, David Kisailus

Research output: Contribution to journalArticle

Abstract

Many species of chiton are known to deposit magnetite (Fe3O4) within the cusps of their heavily mineralized and ultrahard radular teeth. Recently, much attention has been paid to the ultrastructural design and superior mechanical properties of these radular teeth, providing a promising model for the development of novel abrasion resistant materials. Here, we constructed de novo assembled transcripts from the radular tissue of C. stelleri that were used for transcriptome and proteome analysis. Transcriptomic analysis revealed that the top 20 most highly expressed transcripts in the non-mineralized teeth region include the transcripts encoding ferritin, while those in the mineralized teeth region contain a high proportion of mitochondrial respiratory chain proteins. Proteomic analysis identified 22 proteins that were specifically expressed in the mineralized cusp. These specific proteins include a novel protein that we term radular teeth matrix protein1 (RTMP1), globins, peroxidasins, antioxidant enzymes and a ferroxidase protein. This study reports the first de novo transcriptome assembly from C. stelleri, providing a broad overview of radular teeth mineralization. This new transcriptomic resource and the proteomic profiles of mineralized cusp are valuable for further investigation of the molecular mechanisms of radular teeth mineralization in chitons.

Original languageEnglish
Article number856
Pages (from-to)856
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 29 2019

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Proteomics
Tooth
Polyplacophora
Proteins
Ferrosoferric Oxide
Ceruloplasmin
Globins
Gene Expression Profiling
Proteome
Ferritins
Electron Transport
Transcriptome
Antioxidants
Enzymes

ASJC Scopus subject areas

  • General

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Integrated transcriptomic and proteomic analyses of a molecular mechanism of radular teeth biomineralization in Cryptochiton stelleri. / Nemoto, Michiko; Ren, Dongni; Herrera, Steven; Pan, Songqin; Tamura, Takashi; Inagaki, Kenji; Kisailus, David.

In: Scientific Reports, Vol. 9, No. 1, 856, 29.01.2019, p. 856.

Research output: Contribution to journalArticle

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