Insulin secreting cell-laden hydrogel microfiber coated with poly-L-ornithine

Takaichi Watanabe, Teru Okitsu, Marie Shinohara, Sapana Poudel, Yasuyuki Sakai, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes the preparation of insulin secreting (MIN6m9) cell-laden calcium alginate (Ca-Alg) hydrogel microfiber coated with poly-L-ornithine (PLO) using a microfluidic wet-spinning, followed by layer-by-layer (LbL) deposition to enhance the mechanical strength. Both in vitro and in vivo experiments showed that PLO-coated (PLO-Alg) microfiber had higher mechanical strength than Ca-Alg microfiber. In addition, the cells encapsulated in PLO-Alg microfiber after 7 days of culture secreted insulin in response to glucose concentrations. We believe that PLO-Alg microfiber can be useful for the application in robust tissue graft to achieve long-term implantation.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages215-217
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Externally publishedYes
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: Oct 25 2015Oct 29 2015

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period10/25/1510/29/15

Keywords

  • Diabetes mellitus
  • Hydrogel
  • Microfiber

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Watanabe, T., Okitsu, T., Shinohara, M., Poudel, S., Sakai, Y., & Takeuchi, S. (2015). Insulin secreting cell-laden hydrogel microfiber coated with poly-L-ornithine. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 215-217). Chemical and Biological Microsystems Society.