Insulin-like growth factor-II mRNA-binding protein 3 immunocytochemical expression in direct endometrial brushings: Possible diagnostic help in endometrial cytology

Yoshiaki Norimatsu, Kenji Yanoh, Yoshinobu Maeda, Satoshi Irino, Yasuo Hirai, Franco Fulciniti, Tadao K. Kobayashi

Research output: Contribution to journalArticle

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Abstract

Introduction: This study evaluated the immunocytochemical (ICC) expression of IMP3 in direct endometrial brushings processed as liquid-based cytology (LBC) samples of endometrioid adenocarcinoma (EAC), serous carcinoma (ESC) and surface papillary syncytial change (SPSC) with endometrial glandular and stromal breakdown (EGBD) to exploit its possible differential diagnostic aid. Methods: In total, 333 samples of LBC samples were obtained from selected outpatients in parallel with Pipelle endometrial sampling. They consisted of 97 EAC (83 grade 1: EAC-1, 14 EAC-3), 35 ESC and 201 benign endometrial samples (51 proliferative, 42 secretory, 38 atrophic, 70 SPSC with EGBD). ICC expression of insulin-like growth factor-II mRNA-binding protein 3 (IMP3) was manually performed on Papanicolaou-stained LBC samples. Results: The ESC samples showed positive staining cells in 100%, EAC-3 in 28.5%, and EAC-1 in 2.4% cases. All the benign endometrium samples were negative. Only ESC cases showed strong immunoreactivity (≥3+) in more than 50% of tumour cells with an average frequency of 80%. Conclusions: IMP3 is a helpful immunomarker to distinguish ESC from EAC and SPSC in endometrial cytology.

Original languageEnglish
JournalCytopathology
DOIs
Publication statusPublished - Jan 1 2019
Externally publishedYes

Fingerprint

Endometrioid Carcinoma
Insulin-Like Growth Factor II
Cell Biology
Carrier Proteins
Messenger RNA
Carcinoma
Papillary Carcinoma
Endometrium
Outpatients
Staining and Labeling

Keywords

  • endometrial cytology
  • endometrioid adenocarcinoma
  • IMP3 immunocytochemical expression
  • serous carcinoma
  • surface papillary syncytial change

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

Insulin-like growth factor-II mRNA-binding protein 3 immunocytochemical expression in direct endometrial brushings : Possible diagnostic help in endometrial cytology. / Norimatsu, Yoshiaki; Yanoh, Kenji; Maeda, Yoshinobu; Irino, Satoshi; Hirai, Yasuo; Fulciniti, Franco; Kobayashi, Tadao K.

In: Cytopathology, 01.01.2019.

Research output: Contribution to journalArticle

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abstract = "Introduction: This study evaluated the immunocytochemical (ICC) expression of IMP3 in direct endometrial brushings processed as liquid-based cytology (LBC) samples of endometrioid adenocarcinoma (EAC), serous carcinoma (ESC) and surface papillary syncytial change (SPSC) with endometrial glandular and stromal breakdown (EGBD) to exploit its possible differential diagnostic aid. Methods: In total, 333 samples of LBC samples were obtained from selected outpatients in parallel with Pipelle endometrial sampling. They consisted of 97 EAC (83 grade 1: EAC-1, 14 EAC-3), 35 ESC and 201 benign endometrial samples (51 proliferative, 42 secretory, 38 atrophic, 70 SPSC with EGBD). ICC expression of insulin-like growth factor-II mRNA-binding protein 3 (IMP3) was manually performed on Papanicolaou-stained LBC samples. Results: The ESC samples showed positive staining cells in 100{\%}, EAC-3 in 28.5{\%}, and EAC-1 in 2.4{\%} cases. All the benign endometrium samples were negative. Only ESC cases showed strong immunoreactivity (≥3+) in more than 50{\%} of tumour cells with an average frequency of 80{\%}. Conclusions: IMP3 is a helpful immunomarker to distinguish ESC from EAC and SPSC in endometrial cytology.",
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T2 - Possible diagnostic help in endometrial cytology

AU - Norimatsu, Yoshiaki

AU - Yanoh, Kenji

AU - Maeda, Yoshinobu

AU - Irino, Satoshi

AU - Hirai, Yasuo

AU - Fulciniti, Franco

AU - Kobayashi, Tadao K.

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