Insulin-like growth factor-I and its receptor in mouse pituitary glands

Junichi Honda, Sakae Takeuchi, Hiroshi Fukamachi, Sumio Takahashi

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16 Citations (Scopus)

Abstract

Insulin-like growth factor-I (IGF-I) is produced in the liver and other peripheral tissues in response to growth hormone (GH) stimuli. IGF-I regulates diverse physiological functions in an autocrine and/ or paracrine manner. IGF-I and IGF-I receptor (type-I receptor) are expressed in human and rat pituitary glands. However, the cell types of IGF-I-expressing cells and target cells of IGF-I in the pituitary glands are not known. The present study was aimed to identify the cell types of IGF-I-expressing cells and of its type-I receptor-expressing cells in mouse pituitary glands. In the mouse pituitary glands, IGF-I mRNA and IGF-I receptor mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR). IGF-I-expressing cells and its receptor-expressing cells were detected by non-radioisotopic in situ hybridization using mouse IGF-I cDNA and IGF-I receptor cDNA probes, and their cell types were immunocytochemically determined using antibodies raised against pituitary hormones. We found that somatotrophs expressed both IGF-I and IGF-I receptors, and some of corticotrophs expressed IGF-I receptors. Co-localization of IGF-I and GH in the same cultured pituitary cells was observed by dual-labelling immunocytochemistry. The present study demonstrated that pituitary IGF-I produced in somatotrophs regulated functions of somatotrophs and corticotrophs in an autocrine and/or paracrine manner.

Original languageEnglish
Pages (from-to)573-579
Number of pages7
JournalZoological Science
Volume15
Issue number4
Publication statusPublished - Aug 1998

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pituitary gland
insulin-like growth factor I
receptors
mice
cells
somatotropin
pituitary hormones
immunocytochemistry
in situ hybridization
reverse transcriptase polymerase chain reaction
insulin-like growth factor I receptor

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Insulin-like growth factor-I and its receptor in mouse pituitary glands. / Honda, Junichi; Takeuchi, Sakae; Fukamachi, Hiroshi; Takahashi, Sumio.

In: Zoological Science, Vol. 15, No. 4, 08.1998, p. 573-579.

Research output: Contribution to journalArticle

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