Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis

Hideya Fujimoto, Kimiko Itoh, Mikihiro Yamamoto, Junko Kyozuka, Ko Shimamoto

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

As a first step towards development of insect resistant rice we have introduced a truncated δ-endotoxin gene, cryIA(b) of Bacillus thuringiensis (B.t.) which has specific biological activity against lepidopteran insects into a japonica rice. To highly express the cryIA(b) gene in rice the coding sequence was extensively modified based on the codon usage of rice genes. Transgenic plants efficiently expressed the modified cryIA(b) gene at both mRNA and protein levels. Bioassays using R2 generation plants with two major rice insect pests, striped stemborer (Chilo suppressalis) and leaffolder (Cnaphalocrosis medinalis), indicated that transgenic rice plants expressing the CryIA(b) protein are more resistant to these pests than untransformed control plants. Our results suggest that the B.t. endotoxin genes will be useful for the rational development of new rice varieties resistant to major insect pests.

Original languageEnglish
Pages (from-to)1151-1155
Number of pages5
JournalNature Biotechnology
Volume11
Issue number10
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Molecular Medicine
  • Biomedical Engineering

Cite this