Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis

H. Fujimoto, K. Itoh, Mikihiro Yamamoto, J. Kyozuka, K. Shimamoto

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

As a first step towards development of insect resistant rice we have introduced a truncated δ-endotoxin gene, cryIA(b) of Bacillus thuringiensis (B.t.) which has specific biological activity against lepidopteran insects into a japonica rice. To highly express the cryIA(b) gene in rice the coding sequence was extensively modified based on the codon usage of rice genes. Transgenic plants efficiently expressed the modified cryIA(b) gene at both mRNA and protein levels. Bioassays using R2 generation plants with two major rice insect pests, striped stemborer (Chilo suppressalis) and leaffolder (Cnaphalocrosis medinalis), indicated that transgenic rice plants expressing the CryIA(b) protein are more resistant to these pests than untransformed control plants. Our results suggest that the B.t. endotoxin genes will be useful for the rational development of new rice varieties resistant to major insect pests.

Original languageEnglish
Pages (from-to)1151-1155
Number of pages5
JournalNature Biotechnology
Volume11
Issue number10
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Bacillus thuringiensis
Bacilli
Endotoxins
Insects
Genes
Genetically Modified Plants
Proteins
Bioassay
Bioactivity
Pest Control
Oryza
Codon
Biological Assay
Messenger RNA

ASJC Scopus subject areas

  • Microbiology

Cite this

Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis. / Fujimoto, H.; Itoh, K.; Yamamoto, Mikihiro; Kyozuka, J.; Shimamoto, K.

In: Nature Biotechnology, Vol. 11, No. 10, 1993, p. 1151-1155.

Research output: Contribution to journalArticle

Fujimoto, H. ; Itoh, K. ; Yamamoto, Mikihiro ; Kyozuka, J. ; Shimamoto, K. / Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis. In: Nature Biotechnology. 1993 ; Vol. 11, No. 10. pp. 1151-1155.
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