TY - JOUR
T1 - Inositol 1,4,5-Trisphosphate receptor causes formation of ER cisternal stacks in transfected fibroblasts and in cerebellar purkinje cells
AU - Takei, K.
AU - Mignery, G. A.
AU - Mugnaini, E.
AU - Südhof, T. C.
AU - De Camilli, P.
N1 - Funding Information:
We thank Dr. David Bole (Ann Arbor, Ml) for the generous gift of anti-Bip antibodies and Dr. P. McPherson for critically reading the manuscript and for discussions. This grant was supported in part by US-PHS grants to P. D. C. (DK 43078 and MH 45191), T. C. 5. (MH47510), and E. M. (NS 0994). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC Section 1734 solely to indicate fact.
PY - 1994/2
Y1 - 1994/2
N2 - The inositol 1,4,5-trisphosphate receptor (IP3R) is expressed at very high levels in cerebellar Purkinje cells. Within these neurons, it has a widespread distribution throughout the endoplasmic reticulum (ER) and is present at particularly high concentrations at sites of membrane appositions within peculiar stacks of ER cisternae. Here we report that stacks of ER cisternae, reminiscent of those observed in Purkinje cells, can be induced by overexpression of full-length IPA, but not of mutant forms of the protein in COS cells. Within these stacks the IP3R forms a crystalline array at apposed cisternal faces. Additionally, we show that Purkinje cell stacks are not permanent structures. Our findings suggest that massive stack formation in Purkinje cells represents an adaptive response of the ER to hypoxic conditions and is due to the presence of the high concentration of IP3R in its membranes.
AB - The inositol 1,4,5-trisphosphate receptor (IP3R) is expressed at very high levels in cerebellar Purkinje cells. Within these neurons, it has a widespread distribution throughout the endoplasmic reticulum (ER) and is present at particularly high concentrations at sites of membrane appositions within peculiar stacks of ER cisternae. Here we report that stacks of ER cisternae, reminiscent of those observed in Purkinje cells, can be induced by overexpression of full-length IPA, but not of mutant forms of the protein in COS cells. Within these stacks the IP3R forms a crystalline array at apposed cisternal faces. Additionally, we show that Purkinje cell stacks are not permanent structures. Our findings suggest that massive stack formation in Purkinje cells represents an adaptive response of the ER to hypoxic conditions and is due to the presence of the high concentration of IP3R in its membranes.
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U2 - 10.1016/0896-6273(94)90275-5
DO - 10.1016/0896-6273(94)90275-5
M3 - Article
C2 - 8110462
AN - SCOPUS:0028348910
SN - 0896-6273
VL - 12
SP - 327
EP - 342
JO - Neuron
JF - Neuron
IS - 2
ER -