Initial hazard assessment of benzyl salicylate

In vitro genotoxicity test and combined repeated-dose and reproductive/developmental toxicity screening test in rats

Toshime Igarashi, Hiromasa Takashima, Michihito Takabe, Hiroshi Suzuki, Kazuo Ushida, Tomoko Kawamura, Mariko Matsumoto, Takako Iso, Shihori Tanabe, Kaoru Inoue, Atsushi Ono, Takashi Yamada, Akihiko Hirose

Research output: Contribution to journalArticle

Abstract

Benzyl salicylate is used as a fragrance ingredient and an ultraviolet light absorber, but its toxicity is unknown. Therefore, toxicity tests and hazard classification were conducted for screening assessment under the Japanese Chemical Substances Control Law. Benzyl salicylate was found to be non-genotoxic in vitro based on the chromosomal aberration test using Chinese hamster lung cells. However, the combined repeated-dose and reproductive/developmental screening toxicity test, in which male and female rats were administered benzyl salicylate by gavage at 0, 30, 100, or 300 mg/kg/day for 42 and 41–46 days, respectively, from 14 days before mating until postnatal Day 4, showed that repeated doses had major effects on the thymus, liver, epididymis, and femur at 100 and/or 300 mg/kg/day. Furthermore, although benzyl salicylate had no effect on the estrus cycle, fertility, corpus lutea, or implantation rate, embryonic resorption, offspring mortality, and neural tube defects were observed at 300 mg/kg/day, and the offspring had lower body weights at 30 and 100 mg/kg/day, suggesting teratogenicity similar to other salicylates. Based on the developmental toxicity, this chemical was classified as hazard class 2, with a lowest observed adverse effect level (LOAEL) of 30 mg/kg/day and a D-value of 0.003 mg/kg/day.

Original languageEnglish
Pages (from-to)105-117
Number of pages13
JournalRegulatory Toxicology and Pharmacology
Volume100
DOIs
Publication statusPublished - Dec 1 2018

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Mutagenicity Tests
Toxicity Tests
Toxicity
Rats
Hazards
Screening
Fragrances
Thymus
Neural Tube Defects
Salicylates
Epididymis
Corpus Luteum
Estrus
Ultraviolet Rays
Cricetulus
Aberrations
Chromosome Aberrations
Liver
Femur
Thymus Gland

Keywords

  • CAS No. 118-58-1
  • Chemical substances control low
  • D-value
  • Embryonic resorption
  • Existing chemical substance
  • Hazard class
  • Low body weight
  • Neural tube defect
  • Salicylic acid
  • Teratogenic potential

ASJC Scopus subject areas

  • Toxicology

Cite this

Initial hazard assessment of benzyl salicylate : In vitro genotoxicity test and combined repeated-dose and reproductive/developmental toxicity screening test in rats. / Igarashi, Toshime; Takashima, Hiromasa; Takabe, Michihito; Suzuki, Hiroshi; Ushida, Kazuo; Kawamura, Tomoko; Matsumoto, Mariko; Iso, Takako; Tanabe, Shihori; Inoue, Kaoru; Ono, Atsushi; Yamada, Takashi; Hirose, Akihiko.

In: Regulatory Toxicology and Pharmacology, Vol. 100, 01.12.2018, p. 105-117.

Research output: Contribution to journalArticle

Igarashi, T, Takashima, H, Takabe, M, Suzuki, H, Ushida, K, Kawamura, T, Matsumoto, M, Iso, T, Tanabe, S, Inoue, K, Ono, A, Yamada, T & Hirose, A 2018, 'Initial hazard assessment of benzyl salicylate: In vitro genotoxicity test and combined repeated-dose and reproductive/developmental toxicity screening test in rats', Regulatory Toxicology and Pharmacology, vol. 100, pp. 105-117. https://doi.org/10.1016/j.yrtph.2018.10.018
Igarashi, Toshime ; Takashima, Hiromasa ; Takabe, Michihito ; Suzuki, Hiroshi ; Ushida, Kazuo ; Kawamura, Tomoko ; Matsumoto, Mariko ; Iso, Takako ; Tanabe, Shihori ; Inoue, Kaoru ; Ono, Atsushi ; Yamada, Takashi ; Hirose, Akihiko. / Initial hazard assessment of benzyl salicylate : In vitro genotoxicity test and combined repeated-dose and reproductive/developmental toxicity screening test in rats. In: Regulatory Toxicology and Pharmacology. 2018 ; Vol. 100. pp. 105-117.
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AU - Ushida, Kazuo

AU - Kawamura, Tomoko

AU - Matsumoto, Mariko

AU - Iso, Takako

AU - Tanabe, Shihori

AU - Inoue, Kaoru

AU - Ono, Atsushi

AU - Yamada, Takashi

AU - Hirose, Akihiko

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