Initial apical migration of junctional epithelium in rats following application of lipopolysaccharide and proteases

Daisuke Ekuni, Takaaki Tomofuji, Reiko Yamanaka, Kentaro Tachibana, Tatsuo Yamamoto, Tatsuo Watanabe

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Apical migration of junctional epithelium (JE) occurs in association with periodontal pocket formation. The aim of this study was to investigate the gingival changes occurring during apical migration of the JE following application of factors associated with inflammatory periodontal disease pathogenesis. Methods: Six-week-old male Wistar rats were divided into six groups: three experimental groups to investigate gingival changes following 2, 4, and 8 weeks topical application of lipopolysaccharide (LPS) and proteases and three control groups using pyrogen-free water. After 2, 4 or 8 weeks, nuclear DNA fragmentation was detected in periodontal ligament (PDL) fibroblasts using the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method, and proliferative activities of the basal cells and fibroblasts were evaluated through expression of proliferating cell nuclear antigen (PCNA). Collagen destruction was examined histologically. Results: Gingiva treated with LPS and proteases showed an increase in PCNA-positive basal cells but not the fibroblasts. Collagen destruction was observed at 2 weeks; apical migration of the JE and TUNEL-positive fibroblasts was seen at 4 weeks. Conclusions: Following application of LPS and proteases to rat gingival sulci, the apical migration of the JE appears to occur simultaneously with the apoptosis of PDL fibroblasts, which in turn follows proliferation of the basal cells and collagen destruction.

Original languageEnglish
Pages (from-to)43-48
Number of pages6
JournalJournal of Periodontology
Volume76
Issue number1
DOIs
Publication statusPublished - Jan 2005

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Epithelial Attachment
Lipopolysaccharides
Peptide Hydrolases
Fibroblasts
Periodontal Ligament
Collagen
Proliferating Cell Nuclear Antigen
Biotin
Periodontal Pocket
Pyrogens
DNA Nucleotidylexotransferase
Gingiva
Periodontal Diseases
DNA Fragmentation
Transferases
Wistar Rats
Cell Proliferation
Apoptosis
Control Groups
Water

Keywords

  • Animal studies
  • Antigens, nuclear
  • Apical migration
  • Apoptosis
  • Epithelial attachment
  • Fibroblasts, periodontal

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Initial apical migration of junctional epithelium in rats following application of lipopolysaccharide and proteases. / Ekuni, Daisuke; Tomofuji, Takaaki; Yamanaka, Reiko; Tachibana, Kentaro; Yamamoto, Tatsuo; Watanabe, Tatsuo.

In: Journal of Periodontology, Vol. 76, No. 1, 01.2005, p. 43-48.

Research output: Contribution to journalArticle

Ekuni, Daisuke ; Tomofuji, Takaaki ; Yamanaka, Reiko ; Tachibana, Kentaro ; Yamamoto, Tatsuo ; Watanabe, Tatsuo. / Initial apical migration of junctional epithelium in rats following application of lipopolysaccharide and proteases. In: Journal of Periodontology. 2005 ; Vol. 76, No. 1. pp. 43-48.
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