Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity

Kenji Kameda, Takeshi Takaku, Hiromichi Okuda, Yoshiyuki Kimura, Takuo Okuda, Tsutomu Hatano, Isao Agata, Shigeru Arichi

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The leaves of the persimmon Diospyros kaki, have been traditionally used for treatment of hypertensive diseases in Japan. We have studied the inhibitory effects of four flavonoids isolated from the leaves of the persimmon on angiotensin-converting enzyme activity. The four flavonoids astragalin [1], kaempferol-3-O-(2″-O-galloyl)-glucoside [2], isoquercitrin [3], and quercetin-3-O-(2″-O-galloyl)-glucoside [4] inhibited the angiotensin-converting enzyme activity in a dose-dependent fashion. Compounds 1-4 produced 67%, 53%, 33%, and 48% inhibition at a concentration of 300 μg/ml, respectively. The 50% inhibitory concentrations (IC50) of 1 and 2 for the angiotensin-converting enzyme were 180 μg/ml and 280 μg/ml, respectively. On the other hand, 2 and 4 were shown to have tannin activities, but 1 and 3 had no tannin activities. These results suggest that there is no relationship between the inhibition for angiotensin converting enzyme activity and the tannin activity for the four flavonoids.

Original languageEnglish
Pages (from-to)680-683
Number of pages4
JournalJournal of Natural Products
Volume50
Issue number4
Publication statusPublished - 1987

Fingerprint

Diospyros
persimmons
Tannins
peptidyl-dipeptidase A
Enzyme activity
Peptidyl-Dipeptidase A
Flavonoids
flavonoids
Glucosides
enzyme activity
tannins
Inhibitory Concentration 50
glucosides
Enzyme inhibition
inhibitory concentration 50
leaves
Quercetin
isoquercitrin
Diospyros kaki
Japan

ASJC Scopus subject areas

  • Plant Science
  • Chemistry (miscellaneous)
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity. / Kameda, Kenji; Takaku, Takeshi; Okuda, Hiromichi; Kimura, Yoshiyuki; Okuda, Takuo; Hatano, Tsutomu; Agata, Isao; Arichi, Shigeru.

In: Journal of Natural Products, Vol. 50, No. 4, 1987, p. 680-683.

Research output: Contribution to journalArticle

Kameda, K, Takaku, T, Okuda, H, Kimura, Y, Okuda, T, Hatano, T, Agata, I & Arichi, S 1987, 'Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity', Journal of Natural Products, vol. 50, no. 4, pp. 680-683.
Kameda, Kenji ; Takaku, Takeshi ; Okuda, Hiromichi ; Kimura, Yoshiyuki ; Okuda, Takuo ; Hatano, Tsutomu ; Agata, Isao ; Arichi, Shigeru. / Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin-converting enzyme activity. In: Journal of Natural Products. 1987 ; Vol. 50, No. 4. pp. 680-683.
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AU - Kameda, Kenji

AU - Takaku, Takeshi

AU - Okuda, Hiromichi

AU - Kimura, Yoshiyuki

AU - Okuda, Takuo

AU - Hatano, Tsutomu

AU - Agata, Isao

AU - Arichi, Shigeru

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N2 - The leaves of the persimmon Diospyros kaki, have been traditionally used for treatment of hypertensive diseases in Japan. We have studied the inhibitory effects of four flavonoids isolated from the leaves of the persimmon on angiotensin-converting enzyme activity. The four flavonoids astragalin [1], kaempferol-3-O-(2″-O-galloyl)-glucoside [2], isoquercitrin [3], and quercetin-3-O-(2″-O-galloyl)-glucoside [4] inhibited the angiotensin-converting enzyme activity in a dose-dependent fashion. Compounds 1-4 produced 67%, 53%, 33%, and 48% inhibition at a concentration of 300 μg/ml, respectively. The 50% inhibitory concentrations (IC50) of 1 and 2 for the angiotensin-converting enzyme were 180 μg/ml and 280 μg/ml, respectively. On the other hand, 2 and 4 were shown to have tannin activities, but 1 and 3 had no tannin activities. These results suggest that there is no relationship between the inhibition for angiotensin converting enzyme activity and the tannin activity for the four flavonoids.

AB - The leaves of the persimmon Diospyros kaki, have been traditionally used for treatment of hypertensive diseases in Japan. We have studied the inhibitory effects of four flavonoids isolated from the leaves of the persimmon on angiotensin-converting enzyme activity. The four flavonoids astragalin [1], kaempferol-3-O-(2″-O-galloyl)-glucoside [2], isoquercitrin [3], and quercetin-3-O-(2″-O-galloyl)-glucoside [4] inhibited the angiotensin-converting enzyme activity in a dose-dependent fashion. Compounds 1-4 produced 67%, 53%, 33%, and 48% inhibition at a concentration of 300 μg/ml, respectively. The 50% inhibitory concentrations (IC50) of 1 and 2 for the angiotensin-converting enzyme were 180 μg/ml and 280 μg/ml, respectively. On the other hand, 2 and 4 were shown to have tannin activities, but 1 and 3 had no tannin activities. These results suggest that there is no relationship between the inhibition for angiotensin converting enzyme activity and the tannin activity for the four flavonoids.

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