Inhibitory effects of tetrahydrocannabinols and their ten oxygenated metabolites on adenosine triphosphatase activity

M. Arai, K. Watanabe, S. Narimatsu, I. Yamamoto, H. Yoshimura

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of tetrahydrocannabinols (THCs) and their ten metabolites, 11-hydroxy-Δ8-THC, 7α-hydroxy-Δ8-THC, 7β-hydroxy-Δ8-THC, 7-oxo-Δ8-THC, 8α,9α-epoxyhexahydrocannabinol (EHHC), 8β,9β-EHHC, 8α-hydroxy-Δ9-THC, 8β-hydroxy-Δ9-THC, 8-oxo-Δ9-THC and 9α,10α-EHHC, on Na+, K+- and MG2+-adenosine triphosphatase (ATPase) activities were examined using the enzyme from porcine cerebral cortex and mouse brain synaptosomes. Na+,K+-ATPase from porcine cerebral cortex was inhibited by all cannabinoids tested at 10-5 M. All cannabinoids tested except for 8α,9α-EHHC, 8-oxo-Δ9-THC and 9α,10α-EHHC also showed the inhibitory effect on Na+, K+-ATPase activity at 10-5 M of mouse brain synaptosomes. These cannabinoids also inhibited Mg2+ ATPase in the synaptosomes, although the effect was less potent than that on Na+, K+-ATPase. 7-Oxo-Δ8-THC was the most potent inhibitor of ATPases among the metabolites tested. The inhibitory effect of 7-oxo-Δ8-THC is comparable to that of Δ8-THC or Δ9-THC. These results indicate that THC metabolites possess the inhibitory effect on ATPases and may affect the energy production in the biological system.

Original languageEnglish
Pages (from-to)207-217
Number of pages11
JournalResearch Communications in Substances of Abuse
Volume9
Issue number3-4
Publication statusPublished - Jan 1 1988

ASJC Scopus subject areas

  • Medicine (miscellaneous)

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