Inhibitory effects of oenothera biennis (evening primrose) seed extract on streptococcus mutans and S. mutans-induced dental caries in rats

Michiyo Nakano, K. Nagayama, H. Kitagori, K. Fujita, S. Inagaki, Yukiko Takashima, M. Tamesada, S. Kawabata, T. Ooshima

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Oenothera biennis (evening primrose) seed extract (OBSE) is known to contain polyphenols, which may possess antioxidant activities. Polyphenols extracted from several plants are reported to exhibit cariostatic activities by inhibiting mutans streptococcus growth and glucosyltransferase activities. The purpose of the present study was to examine the inhibitory effects of OBSE on the development of dental caries, both in vitro and in vivo. Methods: OBSE was investigated for its inhibitory effects on cellular aggregation, hydrophobicity, sucrose-dependent adherence and insoluble glucan synthesis. Furthermore, biofilm formation was examined in the presence of OBSE, using confocal microscopic imaging. An animal experiment was also performed to examine the in vivo effects. Results: OBSE induced a strong aggregation of Streptococcus mutans MT8148 cells, while cell surface hydrophobicity was decreased by approximately 90% at a concentration of 0.25 mg/ml. The sucrose-dependent adherence of the MT8148 cells was also reduced by addition of OBSE, with a reduction rate of 73% seen at a concentration of 1.00 mg/ml. Additionally, confocal microscopic observations revealed the biofilm development phase to be remarkably changed in the presence of OBSE. Furthermore, insoluble glucan synthesis was significantly reduced when OBSE was present at concentrations greater than 0.03 mg/ml. In an animal experiment, the caries scores in rats given OBSE (0.05 mg/ml in drinking water) were significantly lower than those in rats given water without OBSE. Conclusion: Our results indicate that OBSE has inhibitory activity on dental caries.

Original languageEnglish
Pages (from-to)56-63
Number of pages8
JournalCaries Research
Volume45
Issue number1
DOIs
Publication statusPublished - Apr 2011
Externally publishedYes

Fingerprint

Oenothera biennis
Streptococcus mutans
Dental Caries
Seeds
Glucans
Polyphenols
Biofilms
Hydrophobic and Hydrophilic Interactions
Sucrose
Glucosyltransferases
Drinking Water
Antioxidants
Water
Growth

Keywords

  • Evening primrose
  • Streptococcus mutans

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Inhibitory effects of oenothera biennis (evening primrose) seed extract on streptococcus mutans and S. mutans-induced dental caries in rats. / Nakano, Michiyo; Nagayama, K.; Kitagori, H.; Fujita, K.; Inagaki, S.; Takashima, Yukiko; Tamesada, M.; Kawabata, S.; Ooshima, T.

In: Caries Research, Vol. 45, No. 1, 04.2011, p. 56-63.

Research output: Contribution to journalArticle

Nakano, M, Nagayama, K, Kitagori, H, Fujita, K, Inagaki, S, Takashima, Y, Tamesada, M, Kawabata, S & Ooshima, T 2011, 'Inhibitory effects of oenothera biennis (evening primrose) seed extract on streptococcus mutans and S. mutans-induced dental caries in rats', Caries Research, vol. 45, no. 1, pp. 56-63. https://doi.org/10.1159/000323376
Nakano, Michiyo ; Nagayama, K. ; Kitagori, H. ; Fujita, K. ; Inagaki, S. ; Takashima, Yukiko ; Tamesada, M. ; Kawabata, S. ; Ooshima, T. / Inhibitory effects of oenothera biennis (evening primrose) seed extract on streptococcus mutans and S. mutans-induced dental caries in rats. In: Caries Research. 2011 ; Vol. 45, No. 1. pp. 56-63.
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AU - Fujita, K.

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