Inhibitory effects of (-)-epigallocatechin gallate on the mutation, DNA strand cleavage, and DNA adduct formation by heterocyclic amines

Sakae Arimoto, Naomi Inada, Yasuko Sato, Chitose Sugiyama, Keinosuke Okamoto, Hikoya Hayatsu, Tomoe Negishi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Green tea is known to be a potential chemopreventive agent against cancer. In this study, we investigated the inhibitory activities of tea extracts, and in particular the polyphenolic component (-)-epigallocatechin gallate (EGCG), against heterocyclic amine-induced genotoxicity. The tea extracts displayed inhibition of 2-hydroxyamino-6-methyldipyrido[1,2-a,3′,2′-d]imidazole (Glu-P-1(NHOH)) induced mutagenicity. This inhibition can be accounted for by the presence of EGCG in the extracts. The mutagenic effect of Glu-P-1 (NHOH), which induces single-strand cleavage in supercoiled circular DNA under neutral conditions, was inhibited by EGCG. Using the Drosophila repair test, a test for gross DNA damage, and DNA adduct detection by 32P-postlabeling, we showed that EGCG prevented 2-amino-3,8-dimethylimidazo[4,5-f]quinoline-induced DNA damage and adduct formation in insect DNA. EGCG was found to accelerate the degradation of Glu-P-1(NHOH) in vitro. This observation suggested that the inhibition by EGCG is associated with an accelerated degradation of metabolically activated heterocyclic amines.

Original languageEnglish
Pages (from-to)5150-5153
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume51
Issue number17
DOIs
Publication statusPublished - Aug 13 2003

Fingerprint

heterocyclic amines
DNA adducts
DNA Cleavage
DNA Adducts
epigallocatechin
2-amino-6-methyldipyrido(1,2-a-3',2'-d)imidazole
Amines
mutation
Mutation
DNA
Tea
DNA damage
tea
DNA Damage
extracts
circular DNA
Superhelical DNA
quinoline
Circular DNA
Degradation

Keywords

  • Antimutagenicity
  • DNA adduct
  • DNA strand break
  • Drosophila
  • Epigallocatechin gallate
  • Green tea
  • Heterocyclic amines

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)

Cite this

Inhibitory effects of (-)-epigallocatechin gallate on the mutation, DNA strand cleavage, and DNA adduct formation by heterocyclic amines. / Arimoto, Sakae; Inada, Naomi; Sato, Yasuko; Sugiyama, Chitose; Okamoto, Keinosuke; Hayatsu, Hikoya; Negishi, Tomoe.

In: Journal of Agricultural and Food Chemistry, Vol. 51, No. 17, 13.08.2003, p. 5150-5153.

Research output: Contribution to journalArticle

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