Inhibitory effects of (-)-epigallocatechin gallate on the mutation, DNA strand cleavage, and DNA adduct formation by heterocyclic amines

Sakae Arimoto-Kobayashi, Naomi Inada, Yasuko Sato, Chitose Sugiyama, Keinosuke Okamoto, Hikoya Hayatsu, Tomoe Negishi

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Green tea is known to be a potential chemopreventive agent against cancer. In this study, we investigated the inhibitory activities of tea extracts, and in particular the polyphenolic component (-)-epigallocatechin gallate (EGCG), against heterocyclic amine-induced genotoxicity. The tea extracts displayed inhibition of 2-hydroxyamino-6-methyldipyrido[1,2-a,3′,2′-d]imidazole (Glu-P-1(NHOH)) induced mutagenicity. This inhibition can be accounted for by the presence of EGCG in the extracts. The mutagenic effect of Glu-P-1 (NHOH), which induces single-strand cleavage in supercoiled circular DNA under neutral conditions, was inhibited by EGCG. Using the Drosophila repair test, a test for gross DNA damage, and DNA adduct detection by 32P-postlabeling, we showed that EGCG prevented 2-amino-3,8-dimethylimidazo[4,5-f]quinoline-induced DNA damage and adduct formation in insect DNA. EGCG was found to accelerate the degradation of Glu-P-1(NHOH) in vitro. This observation suggested that the inhibition by EGCG is associated with an accelerated degradation of metabolically activated heterocyclic amines.

Original languageEnglish
Pages (from-to)5150-5153
Number of pages4
JournalJournal of agricultural and food chemistry
Volume51
Issue number17
DOIs
Publication statusPublished - Aug 13 2003

Keywords

  • Antimutagenicity
  • DNA adduct
  • DNA strand break
  • Drosophila
  • Epigallocatechin gallate
  • Green tea
  • Heterocyclic amines

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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