Inhibitory effects of caffeic acid phenethyl ester derivatives on replication of hepatitis C virus

Hui Shen, Atsuya Yamashita, Masamichi Nakakoshi, Hiromasa Yokoe, Masashi Sudo, Hirotake Kasai, Tomohisa Tanaka, Yuusuke Fujimoto, Masanori Ikeda, Nobuyuki Kato, Naoya Sakamoto, Hiroko Shindo, Shinya Maekawa, Nobuyuki Enomoto, Masayoshi Tsubuki, Kohji Moriishi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Caffeic acid phenethyl ester (CAPE) has been reported as a multifunctional compound. In this report, we tested the effect of CAPE and its derivatives on hepatitis C virus (HCV) replication in order to develop an effective anti-HCV compound. CAPE and CAPE derivatives exhibited anti-HCV activity against an HCV replicon cell line of genotype 1b with EC50 values in a range from 1.0 to 109.6 μM. Analyses of chemical structure and antiviral activity suggested that the length of the n-alkyl side chain and catechol moiety are responsible for the anti-HCV activity of these compounds. Caffeic acid n-octyl ester exhibited the highest anti-HCV activity among the tested derivatives with an EC50 value of 1.0 μM and an SI value of 63.1 by using the replicon cell line derived from genotype 1b strain Con1. Treatment with caffeic acid n-octyl ester inhibited HCV replication of genotype 2a at a similar level to that of genotype 1b irrespectively of interferon signaling. Caffeic acid n-octyl ester could synergistically enhance the anti-HCV activities of interferon-alpha 2b, daclatasvir, and VX-222, but neither telaprevir nor danoprevir. These results suggest that caffeic acid n-octyl ester is a potential candidate for novel anti-HCV chemotherapy drugs.

Original languageEnglish
Article numbere82299
JournalPLoS One
Volume8
Issue number12
DOIs
Publication statusPublished - Dec 17 2013

Fingerprint

Hepatitis C virus
caffeic acid
Viruses
Hepacivirus
chemical derivatives
esters
Derivatives
Esters
Genotype
replicon
Replicon
interferon alfa-2b
danoprevir
genotype
Virus Replication
virus replication
Cells
cell lines
caffeic acid phenethyl ester
Cell Line

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Shen, H., Yamashita, A., Nakakoshi, M., Yokoe, H., Sudo, M., Kasai, H., ... Moriishi, K. (2013). Inhibitory effects of caffeic acid phenethyl ester derivatives on replication of hepatitis C virus. PLoS One, 8(12), [e82299]. https://doi.org/10.1371/journal.pone.0082299

Inhibitory effects of caffeic acid phenethyl ester derivatives on replication of hepatitis C virus. / Shen, Hui; Yamashita, Atsuya; Nakakoshi, Masamichi; Yokoe, Hiromasa; Sudo, Masashi; Kasai, Hirotake; Tanaka, Tomohisa; Fujimoto, Yuusuke; Ikeda, Masanori; Kato, Nobuyuki; Sakamoto, Naoya; Shindo, Hiroko; Maekawa, Shinya; Enomoto, Nobuyuki; Tsubuki, Masayoshi; Moriishi, Kohji.

In: PLoS One, Vol. 8, No. 12, e82299, 17.12.2013.

Research output: Contribution to journalArticle

Shen, H, Yamashita, A, Nakakoshi, M, Yokoe, H, Sudo, M, Kasai, H, Tanaka, T, Fujimoto, Y, Ikeda, M, Kato, N, Sakamoto, N, Shindo, H, Maekawa, S, Enomoto, N, Tsubuki, M & Moriishi, K 2013, 'Inhibitory effects of caffeic acid phenethyl ester derivatives on replication of hepatitis C virus', PLoS One, vol. 8, no. 12, e82299. https://doi.org/10.1371/journal.pone.0082299
Shen, Hui ; Yamashita, Atsuya ; Nakakoshi, Masamichi ; Yokoe, Hiromasa ; Sudo, Masashi ; Kasai, Hirotake ; Tanaka, Tomohisa ; Fujimoto, Yuusuke ; Ikeda, Masanori ; Kato, Nobuyuki ; Sakamoto, Naoya ; Shindo, Hiroko ; Maekawa, Shinya ; Enomoto, Nobuyuki ; Tsubuki, Masayoshi ; Moriishi, Kohji. / Inhibitory effects of caffeic acid phenethyl ester derivatives on replication of hepatitis C virus. In: PLoS One. 2013 ; Vol. 8, No. 12.
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