Inhibitory effects of beer on mutation in the ames test and DNA adduct formation in mouse organs induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (PhIP)

Sakae Arimoto-Kobayashi, Rie Ishida, Yumi Nakai, Chiho Idei, Jun Takata, Eizo Takahashi, Keinosuke Okamoto, Tomoe Negishi, Toshimitsu Konuma

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

An evaluation of the antigenotoxic potential of beer components against carcinogens contained in the human diet, namely heterocyclic amines (HCAs) including 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), was determined. The protective mechanism involved was also investigated. Beer samples were found to inhibit the mutagenicity of HCAs in the Ames test. Beer solution, consisting of a freeze-dried and dissolved sample, given as drink-water significantly reduced the formation of PhIP-DNA adducts in mouse colon and lung compared to control mice fed with PhIP in the absence of beer solution. Furthermore, beer solution added in the diet as a food additive mimic significantly reduced the amount of DNA adducts present in the liver and lung of mice fed with PhIP. In an effort to investigate the mechanism responsible for the observed protective effect, the effect of beer solutions on HCA metabolizing enzymes was investigated. Beer solutions inhibited the activity of CYP1A1 and CYP1A2, as determined from deethylation and demethylation assays using 7-ethoxy- and 7-methoxyresolufin, respectively. Considering the overall suppression of PhIP genotoxicity by beer, this study con-firmed that beer components can interfere with the enzyme activity involved in the metabolism of HCAs and subsequently suppress the observed genotoxicity. The results of this study showed that beer components act in a protective capacity against the genotoxic effects of heterocyclic amines in vivo.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume29
Issue number1
DOIs
Publication statusPublished - Jan 2006

Keywords

  • 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)
  • Antigenotoxicity
  • Antimutagenicity
  • Beer
  • DNA adduct
  • In vivo effect

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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