Inhibitory effects of basic or neutral phospholipid on acidic phospholipid-mediated dissociation of adenine nucleotide bound to DnaA protein, the initiator of chromosomal DNA replication

Norikazu Ichihashi, Kenji Kurokawa, Miki Matsuo, Chikara Kaito, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

DnaA protein activity, the initiator of chromosomal DNA replication in bacteria, is regulated by acidic phospholipids such as phosphatidylglycerol (PG) or cardiolipin (CL) via facilitation of the exchange reaction of bound adenine nucleotide. Total lipid isolated from exponentially growing Staphylococcus aureus cells facilitated the release of ATP bound to S. aureus DnaA protein, whereas that from stationary phase cells was inert. Fractionation of total lipid from stationary phase cells revealed that the basic phospholipid, lysylphosphatidyl-glycerol (LPG), inhibited PG- or CL-facilitated release of ATP from DnaA protein. There was an increase in LPG concentration during the stationary phase. A fraction of the total lipid from stationary phase cells of an integrational deletion mprF mutant, in which LPG was lost, facilitated the release of ATP from DnaA protein. A zwitterionic phospholipid, phosphatidylethanolamine, also inhibited PG-facilitated ATP release. These results indicate that interaction of DnaA protein with acidic phospholipids might be regulated by changes in the phospholipid composition of the cell membrane at different growth stages. In addition, the mprF mutant exhibited an increased amount of origin per cell in vivo, suggesting that LPG is involved in regulating the cell cycle event(s).

Original languageEnglish
Pages (from-to)28778-28786
Number of pages9
JournalJournal of Biological Chemistry
Volume278
Issue number31
DOIs
Publication statusPublished - Aug 1 2003
Externally publishedYes

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Adenine Nucleotides
DNA Replication
Phospholipids
Glycerol
Phosphatidylglycerols
Adenosine Triphosphate
DNA
Cardiolipins
Proteins
Lipids
Staphylococcus aureus
Cell membranes
Fractionation
Bacteria
Cell Cycle
Cells
Cell Membrane
Growth
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Inhibitory effects of basic or neutral phospholipid on acidic phospholipid-mediated dissociation of adenine nucleotide bound to DnaA protein, the initiator of chromosomal DNA replication. / Ichihashi, Norikazu; Kurokawa, Kenji; Matsuo, Miki; Kaito, Chikara; Sekimizu, Kazuhisa.

In: Journal of Biological Chemistry, Vol. 278, No. 31, 01.08.2003, p. 28778-28786.

Research output: Contribution to journalArticle

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