Inhibitory effect of pheophorbide α, a chlorophyll-related compound, on skin tumor promotion in ICR mouse

Yoshimasa Nakamura, Akira Murakami, Koichi Koshimizu, Hajime Ohigashi

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Anti-tumor-promoting activity of pheophorbide α (PPBα), a chlorophyll-related compound, was examined in a two-stage carcinogenesis experiment in ICR mouse skin by 7,12-dimethylbenz[a]anthracene (DMBA, O.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA, 1.6 nmol). Topical application of PPBa (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice (inhibitory ratio: IR = 56%, P < 0.01 and 31%, P < 0.005, respectively). PPBα exhibited potent anti-inflammatory activity in ICR mouse ears and moderate inhibitory activity toward TPA-induced superoxide (O2-) generation in differentiated HL-60 cells. While CuPPBa, a synthetic copper complex of PPBα, exhibited higher anti-inflammatory activity than that of indomethacin, it showed little antioxidative effect against formation of lipid hydroperoxides (LOOHs) and malondialdehyde (MDA), suggesting that the antioxidative effect of PPBα might not be important for anti-inflammatory activity. These results imply that the active mechanism of PPBα for anti-tumor promotion might be partly involved in inhibition of TPA-induced inflammatory responses by suppressing leukocyte activation.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalCancer Letters
Volume108
Issue number2
DOIs
Publication statusPublished - Nov 29 1996
Externally publishedYes

Keywords

  • Anti-inflammation
  • Anti-tumor promotion
  • Cancer chemoprevention
  • HL-60
  • Pheophorbidea
  • Reactive oxygen species

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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