Abstract
Anti-tumor-promoting activity of pheophorbide α (PPBα), a chlorophyll-related compound, was examined in a two-stage carcinogenesis experiment in ICR mouse skin by 7,12-dimethylbenz[a]anthracene (DMBA, O.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA, 1.6 nmol). Topical application of PPBa (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice (inhibitory ratio: IR = 56%, P <0.01 and 31%, P <0.005, respectively). PPBα exhibited potent anti-inflammatory activity in ICR mouse ears and moderate inhibitory activity toward TPA-induced superoxide (O2-) generation in differentiated HL-60 cells. While CuPPBa, a synthetic copper complex of PPBα, exhibited higher anti-inflammatory activity than that of indomethacin, it showed little antioxidative effect against formation of lipid hydroperoxides (LOOHs) and malondialdehyde (MDA), suggesting that the antioxidative effect of PPBα might not be important for anti-inflammatory activity. These results imply that the active mechanism of PPBα for anti-tumor promotion might be partly involved in inhibition of TPA-induced inflammatory responses by suppressing leukocyte activation.
| Original language | English |
|---|---|
| Pages (from-to) | 247-255 |
| Number of pages | 9 |
| Journal | Cancer Letters |
| Volume | 108 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Nov 29 1996 |
| Externally published | Yes |
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Keywords
- Anti-inflammation
- Anti-tumor promotion
- Cancer chemoprevention
- HL-60
- Pheophorbidea
- Reactive oxygen species
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
Inhibitory effect of pheophorbide α, a chlorophyll-related compound, on skin tumor promotion in ICR mouse. / Nakamura, Yoshimasa; Murakami, Akira; Koshimizu, Koichi; Ohigashi, Hajime.
In: Cancer Letters, Vol. 108, No. 2, 29.11.1996, p. 247-255.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibitory effect of pheophorbide α, a chlorophyll-related compound, on skin tumor promotion in ICR mouse
AU - Nakamura, Yoshimasa
AU - Murakami, Akira
AU - Koshimizu, Koichi
AU - Ohigashi, Hajime
PY - 1996/11/29
Y1 - 1996/11/29
N2 - Anti-tumor-promoting activity of pheophorbide α (PPBα), a chlorophyll-related compound, was examined in a two-stage carcinogenesis experiment in ICR mouse skin by 7,12-dimethylbenz[a]anthracene (DMBA, O.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA, 1.6 nmol). Topical application of PPBa (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice (inhibitory ratio: IR = 56%, P <0.01 and 31%, P <0.005, respectively). PPBα exhibited potent anti-inflammatory activity in ICR mouse ears and moderate inhibitory activity toward TPA-induced superoxide (O2-) generation in differentiated HL-60 cells. While CuPPBa, a synthetic copper complex of PPBα, exhibited higher anti-inflammatory activity than that of indomethacin, it showed little antioxidative effect against formation of lipid hydroperoxides (LOOHs) and malondialdehyde (MDA), suggesting that the antioxidative effect of PPBα might not be important for anti-inflammatory activity. These results imply that the active mechanism of PPBα for anti-tumor promotion might be partly involved in inhibition of TPA-induced inflammatory responses by suppressing leukocyte activation.
AB - Anti-tumor-promoting activity of pheophorbide α (PPBα), a chlorophyll-related compound, was examined in a two-stage carcinogenesis experiment in ICR mouse skin by 7,12-dimethylbenz[a]anthracene (DMBA, O.19 μmol) and 12-O-tetradecanoylphorbol-13-acetate (TPA, 1.6 nmol). Topical application of PPBa (160 nmol) markedly reduced the average number of tumors per mouse and the ratio of tumor-bearing mice (inhibitory ratio: IR = 56%, P <0.01 and 31%, P <0.005, respectively). PPBα exhibited potent anti-inflammatory activity in ICR mouse ears and moderate inhibitory activity toward TPA-induced superoxide (O2-) generation in differentiated HL-60 cells. While CuPPBa, a synthetic copper complex of PPBα, exhibited higher anti-inflammatory activity than that of indomethacin, it showed little antioxidative effect against formation of lipid hydroperoxides (LOOHs) and malondialdehyde (MDA), suggesting that the antioxidative effect of PPBα might not be important for anti-inflammatory activity. These results imply that the active mechanism of PPBα for anti-tumor promotion might be partly involved in inhibition of TPA-induced inflammatory responses by suppressing leukocyte activation.
KW - Anti-inflammation
KW - Anti-tumor promotion
KW - Cancer chemoprevention
KW - HL-60
KW - Pheophorbidea
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=0030606269&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030606269&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(96)04422-9
DO - 10.1016/S0304-3835(96)04422-9
M3 - Article
C2 - 8973602
AN - SCOPUS:0030606269
VL - 108
SP - 247
EP - 255
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 2
ER -