Inhibitory effect of chlorophyll on the genotoxicity of 3-amino-1-methyl-5h-pyrido[4,3-b indole (trp-p-2)

Tomoe Negishi, Sakae Arimoto, Chiharu Nishizaki, Hikoya Hayatsu

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Genotoxicity of 3-amino-1-methyl-5H-pyrido [4,3-b indole(Trp-P-2) on Drosophila was suppressed by chlorophyll. Using the wing hair spot test, we found that the formation of mutant hairs in adult files as a result of feeding them with Trp-P-2 in their larval stage was efficiently inhibited by coadininistration of chlorophyll. The decrease in the spot frequencies was dependent on the dose of chlorophyll, and at the highest dose used, where the ratio in weight of Trp-P-2 to chlorophyll was 1:80, a complete prevention of the small single-spot formation was observed. A similar inhibitory effect was detected for chlorophyllin, the chromophore of chlorophyll. In the studies to investigate the mechanism of inhibition, we observed that the mutagenicity of 3-hydroxyamino-1-methyl-5H-[4,3-bpyrido [Trp-P-2(NHOH)], the metabolically activated form of Trp-P-2, in Salmonella typhimurium TA98 was suppressed effectively with chlorophyll and chlorophyllin. We also found that chlorophyll and chlorophyllin can produce complexes with Trp-P-2 and Trp-P-2(NHOH). A straightforward mechanism of these inhibitions is that Trp-P-2 [and Trp-P-2(NHOH)] becomes no longer available to organisms on forming the chlorophyll complex.

Original languageEnglish
Pages (from-to)145-149
Number of pages5
JournalCarcinogenesis
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1 1989

ASJC Scopus subject areas

  • Cancer Research

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