Inhibitory effect of chlorophyll on the genotoxicity of 3-amino-1-methyl-5h-pyrido[4,3-b indole (trp-p-2)

Tomoe Negishi, Sakae Arimoto, Chiharu Nishizaki, Hikoya Hayatsu

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Genotoxicity of 3-amino-1-methyl-5H-pyrido [4,3-b indole(Trp-P-2) on Drosophila was suppressed by chlorophyll. Using the wing hair spot test, we found that the formation of mutant hairs in adult files as a result of feeding them with Trp-P-2 in their larval stage was efficiently inhibited by coadininistration of chlorophyll. The decrease in the spot frequencies was dependent on the dose of chlorophyll, and at the highest dose used, where the ratio in weight of Trp-P-2 to chlorophyll was 1:80, a complete prevention of the small single-spot formation was observed. A similar inhibitory effect was detected for chlorophyllin, the chromophore of chlorophyll. In the studies to investigate the mechanism of inhibition, we observed that the mutagenicity of 3-hydroxyamino-1-methyl-5H-[4,3-bpyrido [Trp-P-2(NHOH)], the metabolically activated form of Trp-P-2, in Salmonella typhimurium TA98 was suppressed effectively with chlorophyll and chlorophyllin. We also found that chlorophyll and chlorophyllin can produce complexes with Trp-P-2 and Trp-P-2(NHOH). A straightforward mechanism of these inhibitions is that Trp-P-2 [and Trp-P-2(NHOH)] becomes no longer available to organisms on forming the chlorophyll complex.

Original languageEnglish
Pages (from-to)145-149
Number of pages5
JournalCarcinogenesis
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1989

Fingerprint

Chlorophyll
Dose
Salmonella
3-amino-1-methyl-5H-pyrido(4,3-b)indole
Drosophilidae
Chromophores
Mutant
Salmonella typhimurium
Drosophila
Decrease
Organism
Dependent
Weights and Measures

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology
  • Behavioral Neuroscience
  • Cancer Research

Cite this

Inhibitory effect of chlorophyll on the genotoxicity of 3-amino-1-methyl-5h-pyrido[4,3-b indole (trp-p-2). / Negishi, Tomoe; Arimoto, Sakae; Nishizaki, Chiharu; Hayatsu, Hikoya.

In: Carcinogenesis, Vol. 10, No. 1, 01.1989, p. 145-149.

Research output: Contribution to journalArticle

Negishi, Tomoe ; Arimoto, Sakae ; Nishizaki, Chiharu ; Hayatsu, Hikoya. / Inhibitory effect of chlorophyll on the genotoxicity of 3-amino-1-methyl-5h-pyrido[4,3-b indole (trp-p-2). In: Carcinogenesis. 1989 ; Vol. 10, No. 1. pp. 145-149.
@article{6132e8158e884b689da591c8e8136b92,
title = "Inhibitory effect of chlorophyll on the genotoxicity of 3-amino-1-methyl-5h-pyrido[4,3-b indole (trp-p-2)",
abstract = "Genotoxicity of 3-amino-1-methyl-5H-pyrido [4,3-b indole(Trp-P-2) on Drosophila was suppressed by chlorophyll. Using the wing hair spot test, we found that the formation of mutant hairs in adult files as a result of feeding them with Trp-P-2 in their larval stage was efficiently inhibited by coadininistration of chlorophyll. The decrease in the spot frequencies was dependent on the dose of chlorophyll, and at the highest dose used, where the ratio in weight of Trp-P-2 to chlorophyll was 1:80, a complete prevention of the small single-spot formation was observed. A similar inhibitory effect was detected for chlorophyllin, the chromophore of chlorophyll. In the studies to investigate the mechanism of inhibition, we observed that the mutagenicity of 3-hydroxyamino-1-methyl-5H-[4,3-bpyrido [Trp-P-2(NHOH)], the metabolically activated form of Trp-P-2, in Salmonella typhimurium TA98 was suppressed effectively with chlorophyll and chlorophyllin. We also found that chlorophyll and chlorophyllin can produce complexes with Trp-P-2 and Trp-P-2(NHOH). A straightforward mechanism of these inhibitions is that Trp-P-2 [and Trp-P-2(NHOH)] becomes no longer available to organisms on forming the chlorophyll complex.",
author = "Tomoe Negishi and Sakae Arimoto and Chiharu Nishizaki and Hikoya Hayatsu",
year = "1989",
month = "1",
doi = "10.1093/carcin/10.1.145",
language = "English",
volume = "10",
pages = "145--149",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Inhibitory effect of chlorophyll on the genotoxicity of 3-amino-1-methyl-5h-pyrido[4,3-b indole (trp-p-2)

AU - Negishi, Tomoe

AU - Arimoto, Sakae

AU - Nishizaki, Chiharu

AU - Hayatsu, Hikoya

PY - 1989/1

Y1 - 1989/1

N2 - Genotoxicity of 3-amino-1-methyl-5H-pyrido [4,3-b indole(Trp-P-2) on Drosophila was suppressed by chlorophyll. Using the wing hair spot test, we found that the formation of mutant hairs in adult files as a result of feeding them with Trp-P-2 in their larval stage was efficiently inhibited by coadininistration of chlorophyll. The decrease in the spot frequencies was dependent on the dose of chlorophyll, and at the highest dose used, where the ratio in weight of Trp-P-2 to chlorophyll was 1:80, a complete prevention of the small single-spot formation was observed. A similar inhibitory effect was detected for chlorophyllin, the chromophore of chlorophyll. In the studies to investigate the mechanism of inhibition, we observed that the mutagenicity of 3-hydroxyamino-1-methyl-5H-[4,3-bpyrido [Trp-P-2(NHOH)], the metabolically activated form of Trp-P-2, in Salmonella typhimurium TA98 was suppressed effectively with chlorophyll and chlorophyllin. We also found that chlorophyll and chlorophyllin can produce complexes with Trp-P-2 and Trp-P-2(NHOH). A straightforward mechanism of these inhibitions is that Trp-P-2 [and Trp-P-2(NHOH)] becomes no longer available to organisms on forming the chlorophyll complex.

AB - Genotoxicity of 3-amino-1-methyl-5H-pyrido [4,3-b indole(Trp-P-2) on Drosophila was suppressed by chlorophyll. Using the wing hair spot test, we found that the formation of mutant hairs in adult files as a result of feeding them with Trp-P-2 in their larval stage was efficiently inhibited by coadininistration of chlorophyll. The decrease in the spot frequencies was dependent on the dose of chlorophyll, and at the highest dose used, where the ratio in weight of Trp-P-2 to chlorophyll was 1:80, a complete prevention of the small single-spot formation was observed. A similar inhibitory effect was detected for chlorophyllin, the chromophore of chlorophyll. In the studies to investigate the mechanism of inhibition, we observed that the mutagenicity of 3-hydroxyamino-1-methyl-5H-[4,3-bpyrido [Trp-P-2(NHOH)], the metabolically activated form of Trp-P-2, in Salmonella typhimurium TA98 was suppressed effectively with chlorophyll and chlorophyllin. We also found that chlorophyll and chlorophyllin can produce complexes with Trp-P-2 and Trp-P-2(NHOH). A straightforward mechanism of these inhibitions is that Trp-P-2 [and Trp-P-2(NHOH)] becomes no longer available to organisms on forming the chlorophyll complex.

UR - http://www.scopus.com/inward/record.url?scp=0024587561&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024587561&partnerID=8YFLogxK

U2 - 10.1093/carcin/10.1.145

DO - 10.1093/carcin/10.1.145

M3 - Article

C2 - 2491967

AN - SCOPUS:0024587561

VL - 10

SP - 145

EP - 149

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 1

ER -