Inhibitors of ATP release inhibit vesicular nucleotide transporter

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15 Citations (Scopus)

Abstract

Vesicular nucleotide transporter (VNUT) is responsible for vesicular ATP storage in ATP-secreting cells. In the present study, we examined the effects on VNUT-mediated transport of ATP release inhibitors such as ATP-binding cassette (ABC) proteins, hemichannels, maxi anion channels and P2X7 receptor. The ATP transport activity of proteoliposomes containing purified human VNUT was blocked by glibenclamide, carbenoxolone, 18 á-glycyrrhetinic acid, flufenamic acid, arachidonic acid and A438079 without the formation of Äø (positive inside) as a driving force being affected. Thus, inhibitors of ATP release may inhibit VNUT and subsequent ATP release, since the previous works proved that inhibitors of ATP release blocked VNUT-mediated ATP release at the cell level.

Original languageEnglish
Pages (from-to)1688-1691
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume36
Issue number11
DOIs
Publication statusPublished - Nov 1 2013

Keywords

  • ATP
  • Exocytosis
  • Purinergic chemical transmission
  • Vesicular nucleotide transporter
  • Vesicular storage

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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