Inhibition of vero cell cytotoxic activity in Escherichia coli O157: H7 lysates by globotriaosylceramide, Gb3, from bovine milk

Shinobu Watarai, Tana, Kaoru Inoue, Yasunori Kushi, Emiko Isogai, Kenji Yokota, Katsumi Naka, Keiji Oguma, Hiroshi Kodama

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In order to clarify the presence and verotoxin (VT) inhibitory activity of globotriaosylceramide (Gb3) in bovine milk, we analyzed neutral glycosphingolipids (GSLs) from bovine milk and investigated the inhibitory effect of bovine milk Gb3 on the cytotoxicity of VT2. Five species of neutral GSLs, designated as N-1, N-2, N-3, N-4, and N-5, were separated on thin-layer chromatography (TLC). N-1, N-2, and N-3 showed the same mobility as glucosylceramide, lactosylceramide, and Gb3 on the TLC plate, respectively. N-4 and N-5 GSLs migrated below globoside on the TLC plate. N-3 GSL having the same TLC mobility as Gb3 from bovine milk was immunologically identified as Gb3 by monoclonal antibody against Gb3, anti-CD77 monoclonal antibody. Furthermore, the effect of bovine milk Gb3 on VT2-induced cytotoxicity was investigated. We found that treatment of VT2 with bovine milk Gb3 can reduce the cytotoxic effect of VT2.

Original languageEnglish
Pages (from-to)414-419
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number2
DOIs
Publication statusPublished - Feb 2001

Fingerprint

Vero Cells
Escherichia coli O157
glycosphingolipids
Escherichia coli
Thin layer chromatography
Milk
Thin Layer Chromatography
thin layer chromatography
milk
Neutral Glycosphingolipids
cytotoxicity
Glycosphingolipids
Monoclonal antibodies
cells
Cytotoxicity
monoclonal antibodies
Globosides
Monoclonal Antibodies
Shiga Toxins
Glucosylceramides

Keywords

  • Bovine milk
  • Gb3
  • Glycolipids
  • O-157:H7
  • Verotoxin

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Inhibition of vero cell cytotoxic activity in Escherichia coli O157 : H7 lysates by globotriaosylceramide, Gb3, from bovine milk. / Watarai, Shinobu; Tana; Inoue, Kaoru; Kushi, Yasunori; Isogai, Emiko; Yokota, Kenji; Naka, Katsumi; Oguma, Keiji; Kodama, Hiroshi.

In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 2, 02.2001, p. 414-419.

Research output: Contribution to journalArticle

Watarai, Shinobu ; Tana ; Inoue, Kaoru ; Kushi, Yasunori ; Isogai, Emiko ; Yokota, Kenji ; Naka, Katsumi ; Oguma, Keiji ; Kodama, Hiroshi. / Inhibition of vero cell cytotoxic activity in Escherichia coli O157 : H7 lysates by globotriaosylceramide, Gb3, from bovine milk. In: Bioscience, Biotechnology and Biochemistry. 2001 ; Vol. 65, No. 2. pp. 414-419.
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