Inhibition of tomato yellow leaf curl virus replication by artificial zinc-finger proteins.

Kosuke Takenaka, Yoshihiro Koshino-Kimura, Yasuhiro Aoyama, Takashi Sera

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Previously, we designed an artificial zinc-finger protein (AZP) for blocking a replication protein (Rep) of beet severe curly top virus (BSCTV) from binding to its replication origin and demonstrated that transgenic Arabidopsis plants expressing the AZP are completely resistant to the virus infection. Here we applied the AZP technology to tomato yellow leaf curl virus (TYLCV) infective to an important agricultural crop, tomato. We designed an AZP binding to the direct repeat to block the TYLCV Rep binding and confirmed in gel shift assays that the designed AZP has a higher affinity to the replication origin than that of Rep. Furthermore, we demonstrated in competitive binding assays that the AZP effectively inhibited the Rep binding in vitro. We discuss properties of the AZP for inhibition of TYLCV replication in detail.

Original languageEnglish
Pages (from-to)429-430
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number51
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Zinc Fingers
Lycopersicon esculentum
Virus Replication
Replication Origin
Proteins
Protein Binding
Agricultural Crops
Virus Attachment
Beta vulgaris
Competitive Binding
Nucleic Acid Repetitive Sequences
Genetically Modified Plants
Virus Diseases
Arabidopsis
Gels
Viruses
Technology

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Inhibition of tomato yellow leaf curl virus replication by artificial zinc-finger proteins. / Takenaka, Kosuke; Koshino-Kimura, Yoshihiro; Aoyama, Yasuhiro; Sera, Takashi.

In: Nucleic acids symposium series (2004), No. 51, 2007, p. 429-430.

Research output: Contribution to journalArticle

Takenaka, Kosuke ; Koshino-Kimura, Yoshihiro ; Aoyama, Yasuhiro ; Sera, Takashi. / Inhibition of tomato yellow leaf curl virus replication by artificial zinc-finger proteins. In: Nucleic acids symposium series (2004). 2007 ; No. 51. pp. 429-430.
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