Abstract
The characteristic development of thermotolerance was examined in tsAF8 cells, which were temperature sensitive cell line derived from BHK21, tsAF8 cells lose RNA polymerase II activity and arrest in the G1 phase of the cell cycle at the non-permissive temperature of 39.5-40.6°C. tsAF8 cells were pre-heated at 45°C for 20 min, incubated at 34 or 39.7°C for different durations, and then heated again at 45°C for 30 min. The cells were trypsinized immediately after the second heating to evaluate cell survival. As a result, thermotolerance was developed rapidly in the cells incubated at 34°C. However, the survival rate of the cells incubated at 39.7°C was almost constant. Thermotolerance of BHK21 cells, which were wild-type cells of tsAF8 cells, developed at 39.7°C. These results suggest that thermotolerance may be inhibited in tsAF8 cells incubated at 39.7°C, and that some metabolism which is inhibited in tsAF8 cells at 39.7°C, may be related to thermotolerance development.
| Original language | English |
|---|---|
| Pages (from-to) | 449-456 |
| Number of pages | 8 |
| Journal | International Journal of Hyperthermia |
| Volume | 16 |
| Issue number | 5 |
| Publication status | Published - 2000 |
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Keywords
- BHK21
- Hyperthermia
- Thermotolerance
- tsAF8
ASJC Scopus subject areas
- Cancer Research
- Radiological and Ultrasound Technology
Cite this
Inhibition of thermotolerance observed on tsAF8 cells. / Shibuya, Koichi; Kawasaki, S.; Kuroda, Masahiro; Asaumi, Jun-Ichi; Hiraki, Y.
In: International Journal of Hyperthermia, Vol. 16, No. 5, 2000, p. 449-456.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of thermotolerance observed on tsAF8 cells
AU - Shibuya, Koichi
AU - Kawasaki, S.
AU - Kuroda, Masahiro
AU - Asaumi, Jun-Ichi
AU - Hiraki, Y.
PY - 2000
Y1 - 2000
N2 - The characteristic development of thermotolerance was examined in tsAF8 cells, which were temperature sensitive cell line derived from BHK21, tsAF8 cells lose RNA polymerase II activity and arrest in the G1 phase of the cell cycle at the non-permissive temperature of 39.5-40.6°C. tsAF8 cells were pre-heated at 45°C for 20 min, incubated at 34 or 39.7°C for different durations, and then heated again at 45°C for 30 min. The cells were trypsinized immediately after the second heating to evaluate cell survival. As a result, thermotolerance was developed rapidly in the cells incubated at 34°C. However, the survival rate of the cells incubated at 39.7°C was almost constant. Thermotolerance of BHK21 cells, which were wild-type cells of tsAF8 cells, developed at 39.7°C. These results suggest that thermotolerance may be inhibited in tsAF8 cells incubated at 39.7°C, and that some metabolism which is inhibited in tsAF8 cells at 39.7°C, may be related to thermotolerance development.
AB - The characteristic development of thermotolerance was examined in tsAF8 cells, which were temperature sensitive cell line derived from BHK21, tsAF8 cells lose RNA polymerase II activity and arrest in the G1 phase of the cell cycle at the non-permissive temperature of 39.5-40.6°C. tsAF8 cells were pre-heated at 45°C for 20 min, incubated at 34 or 39.7°C for different durations, and then heated again at 45°C for 30 min. The cells were trypsinized immediately after the second heating to evaluate cell survival. As a result, thermotolerance was developed rapidly in the cells incubated at 34°C. However, the survival rate of the cells incubated at 39.7°C was almost constant. Thermotolerance of BHK21 cells, which were wild-type cells of tsAF8 cells, developed at 39.7°C. These results suggest that thermotolerance may be inhibited in tsAF8 cells incubated at 39.7°C, and that some metabolism which is inhibited in tsAF8 cells at 39.7°C, may be related to thermotolerance development.
KW - BHK21
KW - Hyperthermia
KW - Thermotolerance
KW - tsAF8
UR - http://www.scopus.com/inward/record.url?scp=0033811534&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033811534&partnerID=8YFLogxK
M3 - Article
C2 - 11001579
AN - SCOPUS:0033811534
VL - 16
SP - 449
EP - 456
JO - International Journal of Hyperthermia
JF - International Journal of Hyperthermia
SN - 0265-6736
IS - 5
ER -