Inhibition of thermotolerance observed on tsAF8 cells

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The characteristic development of thermotolerance was examined in tsAF8 cells, which were temperature sensitive cell line derived from BHK21, tsAF8 cells lose RNA polymerase II activity and arrest in the G1 phase of the cell cycle at the non-permissive temperature of 39.5-40.6°C. tsAF8 cells were pre-heated at 45°C for 20 min, incubated at 34 or 39.7°C for different durations, and then heated again at 45°C for 30 min. The cells were trypsinized immediately after the second heating to evaluate cell survival. As a result, thermotolerance was developed rapidly in the cells incubated at 34°C. However, the survival rate of the cells incubated at 39.7°C was almost constant. Thermotolerance of BHK21 cells, which were wild-type cells of tsAF8 cells, developed at 39.7°C. These results suggest that thermotolerance may be inhibited in tsAF8 cells incubated at 39.7°C, and that some metabolism which is inhibited in tsAF8 cells at 39.7°C, may be related to thermotolerance development.

Original languageEnglish
Pages (from-to)449-456
Number of pages8
JournalInternational Journal of Hyperthermia
Volume16
Issue number5
Publication statusPublished - 2000

Fingerprint

Thermotolerance
Temperature
RNA Polymerase II
G1 Phase
Heating
Cell Survival
Cell Cycle
Cell Line

Keywords

  • BHK21
  • Hyperthermia
  • Thermotolerance
  • tsAF8

ASJC Scopus subject areas

  • Cancer Research
  • Radiological and Ultrasound Technology

Cite this

Inhibition of thermotolerance observed on tsAF8 cells. / Shibuya, Koichi; Kawasaki, S.; Kuroda, Masahiro; Asaumi, Jun-Ichi; Hiraki, Y.

In: International Journal of Hyperthermia, Vol. 16, No. 5, 2000, p. 449-456.

Research output: Contribution to journalArticle

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AU - Kawasaki, S.

AU - Kuroda, Masahiro

AU - Asaumi, Jun-Ichi

AU - Hiraki, Y.

PY - 2000

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N2 - The characteristic development of thermotolerance was examined in tsAF8 cells, which were temperature sensitive cell line derived from BHK21, tsAF8 cells lose RNA polymerase II activity and arrest in the G1 phase of the cell cycle at the non-permissive temperature of 39.5-40.6°C. tsAF8 cells were pre-heated at 45°C for 20 min, incubated at 34 or 39.7°C for different durations, and then heated again at 45°C for 30 min. The cells were trypsinized immediately after the second heating to evaluate cell survival. As a result, thermotolerance was developed rapidly in the cells incubated at 34°C. However, the survival rate of the cells incubated at 39.7°C was almost constant. Thermotolerance of BHK21 cells, which were wild-type cells of tsAF8 cells, developed at 39.7°C. These results suggest that thermotolerance may be inhibited in tsAF8 cells incubated at 39.7°C, and that some metabolism which is inhibited in tsAF8 cells at 39.7°C, may be related to thermotolerance development.

AB - The characteristic development of thermotolerance was examined in tsAF8 cells, which were temperature sensitive cell line derived from BHK21, tsAF8 cells lose RNA polymerase II activity and arrest in the G1 phase of the cell cycle at the non-permissive temperature of 39.5-40.6°C. tsAF8 cells were pre-heated at 45°C for 20 min, incubated at 34 or 39.7°C for different durations, and then heated again at 45°C for 30 min. The cells were trypsinized immediately after the second heating to evaluate cell survival. As a result, thermotolerance was developed rapidly in the cells incubated at 34°C. However, the survival rate of the cells incubated at 39.7°C was almost constant. Thermotolerance of BHK21 cells, which were wild-type cells of tsAF8 cells, developed at 39.7°C. These results suggest that thermotolerance may be inhibited in tsAF8 cells incubated at 39.7°C, and that some metabolism which is inhibited in tsAF8 cells at 39.7°C, may be related to thermotolerance development.

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