Inhibition of sulfate-forming activity in rat liver mitochondria by (aminooxy)acetate

T. Teraoka, J. Ohta, T. Abe, H. Inoue, T. Ubuka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effect of (aminooxy)acetate, an inhibitor of aminotransferases, on the sulfate formation from l-cysteine and l-cysteinesulfinate in rat liver mitochondria was studied. Incubation of 10 mM l-cysteine with rat liver mitochondria at 37°C in the presence of 10 mM 2-oxoglutarate and 10 mM glutathione resulted in the formation of 4.60 and 1.52 μmol of sulfate and thiosulfate, respectively, per 60 min per mitochondria obtained from 1 g of liver. Under the same conditions sulfate formation from l-cysteinesulfinate was 24.96 μmol, but thiosulfate was not formed. The addition of (aminooxy)acetate at 2 mM or more completely inhibited the sulfate and thiosulfate formation from l-cysteine and the sulfate formation from l-cysteinesulfinate. These findings support our previous conclusion that cysteine transamination and 3-mercaptopyruvate pathway (MP pathway) are involved in the sulfate formation from l-cysteine in rat liver mitochondria (Ubuka et al., 1992).

Original languageEnglish
Pages (from-to)245-251
Number of pages7
JournalAmino Acids
Volume5
Issue number2
DOIs
Publication statusPublished - Jun 1 1993

Keywords

  • (Aminooxy)acetate
  • Amino acids
  • Cysteine metabolism
  • MP pathway
  • Rat liver mitochondria
  • Sulfate formation

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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