Inhibition of RL male 1 tumor growth in BALB/c mice by introduction of the RLakt gene coding for antigen recognized by cytotoxic T-lymphocytes and the GM-CSF gene by in vivo electroporation

Motoyuki Tanaka, Masaharu Yamada, Toshiro Ono, Yuji Naguchi, Akiko Uenaka, Seisuke Ota, Hidenori Hata, Mine Harada, Mitsune Tanimoto, Eiichi Nakayama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A DNA vaccine for inducing a tumor immune response was investigated using a well-characterized murine model tumor antigen. We demonstrated that in vivo electroporation augmented the induction of IFNγ enzyme-linked immunospot (ELISPOT) and cytotoxic T lymphocyte (CTL) generation against pRL1a peptide in BALB/c spleen cells upon immunization with RLakt plasmid. Immunization without in vivo electroporation resulted in only a marginal induction of IFNγ ELISPOT and CTL generation. Furthermore, co-injection of GM-CSF and RLakt plasmids significantly enhanced the induction of IFNγ ELISPOT and CTL generation compared to the injection of RLakt plasmid alone. Inhibition of RL male 1 tumor growth was observed by injecting BALB/c mice with GM-CSF and RLakt plasmids using in vivo electroporation, although no effect was observed against an established tumor using the same treatment. No growth inhibition was observed without in vivo electroporation. Immunization with either RLakt plasmid alone, or GM-CSF and pCIneo control plasmids using in vivo electroporation did not inhibit RL male 1 tumor growth.

Original languageEnglish
Pages (from-to)154-159
Number of pages6
JournalCancer Science
Volume95
Issue number2
DOIs
Publication statusPublished - Feb 2004

Fingerprint

Electroporation
Cytotoxic T-Lymphocytes
Granulocyte-Macrophage Colony-Stimulating Factor
Plasmids
Antigens
Enzyme Induction
Growth
Genes
Immunization
Neoplasms
Injections
DNA Vaccines
Neoplasm Antigens
Spleen
Peptides

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Inhibition of RL male 1 tumor growth in BALB/c mice by introduction of the RLakt gene coding for antigen recognized by cytotoxic T-lymphocytes and the GM-CSF gene by in vivo electroporation. / Tanaka, Motoyuki; Yamada, Masaharu; Ono, Toshiro; Naguchi, Yuji; Uenaka, Akiko; Ota, Seisuke; Hata, Hidenori; Harada, Mine; Tanimoto, Mitsune; Nakayama, Eiichi.

In: Cancer Science, Vol. 95, No. 2, 02.2004, p. 154-159.

Research output: Contribution to journalArticle

Tanaka, Motoyuki ; Yamada, Masaharu ; Ono, Toshiro ; Naguchi, Yuji ; Uenaka, Akiko ; Ota, Seisuke ; Hata, Hidenori ; Harada, Mine ; Tanimoto, Mitsune ; Nakayama, Eiichi. / Inhibition of RL male 1 tumor growth in BALB/c mice by introduction of the RLakt gene coding for antigen recognized by cytotoxic T-lymphocytes and the GM-CSF gene by in vivo electroporation. In: Cancer Science. 2004 ; Vol. 95, No. 2. pp. 154-159.
@article{ed3355c2b2a04facae24129e8757ed4a,
title = "Inhibition of RL male 1 tumor growth in BALB/c mice by introduction of the RLakt gene coding for antigen recognized by cytotoxic T-lymphocytes and the GM-CSF gene by in vivo electroporation",
abstract = "A DNA vaccine for inducing a tumor immune response was investigated using a well-characterized murine model tumor antigen. We demonstrated that in vivo electroporation augmented the induction of IFNγ enzyme-linked immunospot (ELISPOT) and cytotoxic T lymphocyte (CTL) generation against pRL1a peptide in BALB/c spleen cells upon immunization with RLakt plasmid. Immunization without in vivo electroporation resulted in only a marginal induction of IFNγ ELISPOT and CTL generation. Furthermore, co-injection of GM-CSF and RLakt plasmids significantly enhanced the induction of IFNγ ELISPOT and CTL generation compared to the injection of RLakt plasmid alone. Inhibition of RL male 1 tumor growth was observed by injecting BALB/c mice with GM-CSF and RLakt plasmids using in vivo electroporation, although no effect was observed against an established tumor using the same treatment. No growth inhibition was observed without in vivo electroporation. Immunization with either RLakt plasmid alone, or GM-CSF and pCIneo control plasmids using in vivo electroporation did not inhibit RL male 1 tumor growth.",
author = "Motoyuki Tanaka and Masaharu Yamada and Toshiro Ono and Yuji Naguchi and Akiko Uenaka and Seisuke Ota and Hidenori Hata and Mine Harada and Mitsune Tanimoto and Eiichi Nakayama",
year = "2004",
month = "2",
doi = "10.1111/j.1349-7006.2004.tb03197.x",
language = "English",
volume = "95",
pages = "154--159",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Inhibition of RL male 1 tumor growth in BALB/c mice by introduction of the RLakt gene coding for antigen recognized by cytotoxic T-lymphocytes and the GM-CSF gene by in vivo electroporation

AU - Tanaka, Motoyuki

AU - Yamada, Masaharu

AU - Ono, Toshiro

AU - Naguchi, Yuji

AU - Uenaka, Akiko

AU - Ota, Seisuke

AU - Hata, Hidenori

AU - Harada, Mine

AU - Tanimoto, Mitsune

AU - Nakayama, Eiichi

PY - 2004/2

Y1 - 2004/2

N2 - A DNA vaccine for inducing a tumor immune response was investigated using a well-characterized murine model tumor antigen. We demonstrated that in vivo electroporation augmented the induction of IFNγ enzyme-linked immunospot (ELISPOT) and cytotoxic T lymphocyte (CTL) generation against pRL1a peptide in BALB/c spleen cells upon immunization with RLakt plasmid. Immunization without in vivo electroporation resulted in only a marginal induction of IFNγ ELISPOT and CTL generation. Furthermore, co-injection of GM-CSF and RLakt plasmids significantly enhanced the induction of IFNγ ELISPOT and CTL generation compared to the injection of RLakt plasmid alone. Inhibition of RL male 1 tumor growth was observed by injecting BALB/c mice with GM-CSF and RLakt plasmids using in vivo electroporation, although no effect was observed against an established tumor using the same treatment. No growth inhibition was observed without in vivo electroporation. Immunization with either RLakt plasmid alone, or GM-CSF and pCIneo control plasmids using in vivo electroporation did not inhibit RL male 1 tumor growth.

AB - A DNA vaccine for inducing a tumor immune response was investigated using a well-characterized murine model tumor antigen. We demonstrated that in vivo electroporation augmented the induction of IFNγ enzyme-linked immunospot (ELISPOT) and cytotoxic T lymphocyte (CTL) generation against pRL1a peptide in BALB/c spleen cells upon immunization with RLakt plasmid. Immunization without in vivo electroporation resulted in only a marginal induction of IFNγ ELISPOT and CTL generation. Furthermore, co-injection of GM-CSF and RLakt plasmids significantly enhanced the induction of IFNγ ELISPOT and CTL generation compared to the injection of RLakt plasmid alone. Inhibition of RL male 1 tumor growth was observed by injecting BALB/c mice with GM-CSF and RLakt plasmids using in vivo electroporation, although no effect was observed against an established tumor using the same treatment. No growth inhibition was observed without in vivo electroporation. Immunization with either RLakt plasmid alone, or GM-CSF and pCIneo control plasmids using in vivo electroporation did not inhibit RL male 1 tumor growth.

UR - http://www.scopus.com/inward/record.url?scp=12144288305&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=12144288305&partnerID=8YFLogxK

U2 - 10.1111/j.1349-7006.2004.tb03197.x

DO - 10.1111/j.1349-7006.2004.tb03197.x

M3 - Article

VL - 95

SP - 154

EP - 159

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 2

ER -