Inhibition of placental ornithine decarboxylase by DL-α-difluoro-methyl ornithine causes fetal growth restriction in rat

Makoto Ishida, Yuji Hiramatsu, Hisashi Masuyama, Yasushi Mizutani, Takafumi Kudo

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The roles of polyamines in intrauterine growth restriction (IUGR) is studied. The DL-α-difluoromethyl ornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC) which is a rate limiting enzyme of polyamine synthesis was administrated to pregnant rats so that we obtained rat fetuses with IUGR. The changes of maternal nutrition, damage of the placenta, and the direct effect of DFMO on the fetus were examined in this IUGR model. Administration of DFMO did not induced changes of maternal nutrition except for triglyceride and the fetal metabolic state. But the placental weight, ODC activity, and DNA in the placenta were decreased significantly. The ODC activity in the total placenta decreased to less than 10% of that of the control. Depression of ODC activity in the placenta may be the major cause of IUGR induced by DFMO administration, and polyamines play important roles to carry pregnancy.

Original languageEnglish
Pages (from-to)1395-1405
Number of pages11
JournalLife Sciences
Volume70
Issue number12
DOIs
Publication statusPublished - Feb 8 2002

Fingerprint

Ornithine
Ornithine Decarboxylase
Fetal Development
Placenta
Rats
Polyamines
Nutrition
Growth
Fetus
Mothers
Triglycerides
Weights and Measures
Pregnancy
DNA
Enzymes

Keywords

  • DL-α-difluoromethyl ornithine
  • Intrauterine growth restriction
  • Ornithine decarboxylase
  • Rat

ASJC Scopus subject areas

  • Pharmacology

Cite this

Inhibition of placental ornithine decarboxylase by DL-α-difluoro-methyl ornithine causes fetal growth restriction in rat. / Ishida, Makoto; Hiramatsu, Yuji; Masuyama, Hisashi; Mizutani, Yasushi; Kudo, Takafumi.

In: Life Sciences, Vol. 70, No. 12, 08.02.2002, p. 1395-1405.

Research output: Contribution to journalArticle

Ishida, Makoto ; Hiramatsu, Yuji ; Masuyama, Hisashi ; Mizutani, Yasushi ; Kudo, Takafumi. / Inhibition of placental ornithine decarboxylase by DL-α-difluoro-methyl ornithine causes fetal growth restriction in rat. In: Life Sciences. 2002 ; Vol. 70, No. 12. pp. 1395-1405.
@article{2b70ecb224094413a872a4280d8d3601,
title = "Inhibition of placental ornithine decarboxylase by DL-α-difluoro-methyl ornithine causes fetal growth restriction in rat",
abstract = "The roles of polyamines in intrauterine growth restriction (IUGR) is studied. The DL-α-difluoromethyl ornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC) which is a rate limiting enzyme of polyamine synthesis was administrated to pregnant rats so that we obtained rat fetuses with IUGR. The changes of maternal nutrition, damage of the placenta, and the direct effect of DFMO on the fetus were examined in this IUGR model. Administration of DFMO did not induced changes of maternal nutrition except for triglyceride and the fetal metabolic state. But the placental weight, ODC activity, and DNA in the placenta were decreased significantly. The ODC activity in the total placenta decreased to less than 10{\%} of that of the control. Depression of ODC activity in the placenta may be the major cause of IUGR induced by DFMO administration, and polyamines play important roles to carry pregnancy.",
keywords = "DL-α-difluoromethyl ornithine, Intrauterine growth restriction, Ornithine decarboxylase, Rat",
author = "Makoto Ishida and Yuji Hiramatsu and Hisashi Masuyama and Yasushi Mizutani and Takafumi Kudo",
year = "2002",
month = "2",
day = "8",
doi = "10.1016/S0024-3205(01)01510-7",
language = "English",
volume = "70",
pages = "1395--1405",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "12",

}

TY - JOUR

T1 - Inhibition of placental ornithine decarboxylase by DL-α-difluoro-methyl ornithine causes fetal growth restriction in rat

AU - Ishida, Makoto

AU - Hiramatsu, Yuji

AU - Masuyama, Hisashi

AU - Mizutani, Yasushi

AU - Kudo, Takafumi

PY - 2002/2/8

Y1 - 2002/2/8

N2 - The roles of polyamines in intrauterine growth restriction (IUGR) is studied. The DL-α-difluoromethyl ornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC) which is a rate limiting enzyme of polyamine synthesis was administrated to pregnant rats so that we obtained rat fetuses with IUGR. The changes of maternal nutrition, damage of the placenta, and the direct effect of DFMO on the fetus were examined in this IUGR model. Administration of DFMO did not induced changes of maternal nutrition except for triglyceride and the fetal metabolic state. But the placental weight, ODC activity, and DNA in the placenta were decreased significantly. The ODC activity in the total placenta decreased to less than 10% of that of the control. Depression of ODC activity in the placenta may be the major cause of IUGR induced by DFMO administration, and polyamines play important roles to carry pregnancy.

AB - The roles of polyamines in intrauterine growth restriction (IUGR) is studied. The DL-α-difluoromethyl ornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC) which is a rate limiting enzyme of polyamine synthesis was administrated to pregnant rats so that we obtained rat fetuses with IUGR. The changes of maternal nutrition, damage of the placenta, and the direct effect of DFMO on the fetus were examined in this IUGR model. Administration of DFMO did not induced changes of maternal nutrition except for triglyceride and the fetal metabolic state. But the placental weight, ODC activity, and DNA in the placenta were decreased significantly. The ODC activity in the total placenta decreased to less than 10% of that of the control. Depression of ODC activity in the placenta may be the major cause of IUGR induced by DFMO administration, and polyamines play important roles to carry pregnancy.

KW - DL-α-difluoromethyl ornithine

KW - Intrauterine growth restriction

KW - Ornithine decarboxylase

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=0037040036&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037040036&partnerID=8YFLogxK

U2 - 10.1016/S0024-3205(01)01510-7

DO - 10.1016/S0024-3205(01)01510-7

M3 - Article

VL - 70

SP - 1395

EP - 1405

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 12

ER -