Inhibition of human papillomavirus replication by using artificial zinc-finger nucleases.

Takashi Mino, Tomoaki Mori, Yasuhiro Aoyama, Takashi Sera

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Recently, we have designed artificial zinc-finger proteins (AZPs) that prevent a viral replication protein, E2, of human papillomavirus type 18 (HPV-18) from binding to its replication origin and demonstrated that the gene-delivered AZPs inhibited HPV-18 DNA replication in mammalian cells. In the present study, we examined a new approach to inhibition of DNA virus replication by using an AZP-nuclease fusion. In transient replication assays for HPV-18, the gene-delivered AZP-nuclease fusion reduced the viral DNA replication rate significantly. Moreover, it was demonstrated by ligation-mediated PCR that viral DNA regions close to the AZP-binding site were cleaved in the cells by the AZP-nuclease. Thus, our results demonstrate that AZP-nucleases have potentials to inhibit replication of any DNA viruses whose replication mechanisms remain unsolved.

Original languageEnglish
Pages (from-to)185-186
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number52
Publication statusPublished - Dec 1 2008
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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