Inhibition of herpes simplex virus infection by tannins and related compounds

Kunihiko Fukuchi, Hiroshi Sakagami, Takuo Okuda, Tsutomu Hatano, Sei ichi Tanuma, Ken Kitajima, Yasuo Inoue, Sadako Inoue, Shinya Ichikawa, Meihan Nonoyama, Kunio Konno

Research output: Contribution to journalArticlepeer-review

180 Citations (Scopus)


Several chemically defined plant extracts were investigated for their antiviral action on herpes simplex virus (HSV-1, HSV-2)-infected African green monkey kidney cells and human adenocarcinoma cells, using a plaque formation assay. Among them, the monomeric hydrolyzable tannins, oligomeric ellagitannins and condensed tannins, having galloyl groups or hexahydroxydiphenoyl groups, had the most potent anti-HSV activity. Their 50% effective doses (0.03-0.1 μg/ml) were by two-three orders of magnitude lower than their 50% cytotoxic doses (> 10 μg/ml). On the other hand, gallic acid, neutral polysaccharides, chemically modified (N,N-dimethylaminoethyl-, carboxymethyl-, and sulfated-) glucans, sialic acid-rich glycoproteins, and uronic acid-rich pine cone polysaccharide showed little or no activity. Using radiolabeled virus particles, we demonstrated that the anti-HSV effect of the tannins is due to inhibition of virus adsorption to the cells.

Original languageEnglish
Pages (from-to)285-297
Number of pages13
JournalAntiviral Research
Issue number5-6
Publication statusPublished - 1989


  • Herpes simplex virus
  • Tannin
  • Virus adsorption

ASJC Scopus subject areas

  • Pharmacology
  • Virology


Dive into the research topics of 'Inhibition of herpes simplex virus infection by tannins and related compounds'. Together they form a unique fingerprint.

Cite this