TY - JOUR
T1 - Inhibition of herpes simplex virus infection by tannins and related compounds
AU - Fukuchi, Kunihiko
AU - Sakagami, Hiroshi
AU - Okuda, Takuo
AU - Hatano, Tsutomu
AU - Tanuma, Sei ichi
AU - Kitajima, Ken
AU - Inoue, Yasuo
AU - Inoue, Sadako
AU - Ichikawa, Shinya
AU - Nonoyama, Meihan
AU - Konno, Kunio
N1 - Funding Information:
We thank Dr. K. Hirai for valuable discussions, and Dr. A. Simpson for help with the manuscript. This study was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan, and the SHORAI Foundation for Science and Technology.
PY - 1989
Y1 - 1989
N2 - Several chemically defined plant extracts were investigated for their antiviral action on herpes simplex virus (HSV-1, HSV-2)-infected African green monkey kidney cells and human adenocarcinoma cells, using a plaque formation assay. Among them, the monomeric hydrolyzable tannins, oligomeric ellagitannins and condensed tannins, having galloyl groups or hexahydroxydiphenoyl groups, had the most potent anti-HSV activity. Their 50% effective doses (0.03-0.1 μg/ml) were by two-three orders of magnitude lower than their 50% cytotoxic doses (> 10 μg/ml). On the other hand, gallic acid, neutral polysaccharides, chemically modified (N,N-dimethylaminoethyl-, carboxymethyl-, and sulfated-) glucans, sialic acid-rich glycoproteins, and uronic acid-rich pine cone polysaccharide showed little or no activity. Using radiolabeled virus particles, we demonstrated that the anti-HSV effect of the tannins is due to inhibition of virus adsorption to the cells.
AB - Several chemically defined plant extracts were investigated for their antiviral action on herpes simplex virus (HSV-1, HSV-2)-infected African green monkey kidney cells and human adenocarcinoma cells, using a plaque formation assay. Among them, the monomeric hydrolyzable tannins, oligomeric ellagitannins and condensed tannins, having galloyl groups or hexahydroxydiphenoyl groups, had the most potent anti-HSV activity. Their 50% effective doses (0.03-0.1 μg/ml) were by two-three orders of magnitude lower than their 50% cytotoxic doses (> 10 μg/ml). On the other hand, gallic acid, neutral polysaccharides, chemically modified (N,N-dimethylaminoethyl-, carboxymethyl-, and sulfated-) glucans, sialic acid-rich glycoproteins, and uronic acid-rich pine cone polysaccharide showed little or no activity. Using radiolabeled virus particles, we demonstrated that the anti-HSV effect of the tannins is due to inhibition of virus adsorption to the cells.
KW - Herpes simplex virus
KW - Tannin
KW - Virus adsorption
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U2 - 10.1016/0166-3542(89)90038-7
DO - 10.1016/0166-3542(89)90038-7
M3 - Article
C2 - 2552915
AN - SCOPUS:0024322143
SN - 0166-3542
VL - 11
SP - 285
EP - 297
JO - Antiviral Research
JF - Antiviral Research
IS - 5-6
ER -