Inhibition of exotoxin production by mobile genetic element SCC mec-encoded psm-mec RNA is conserved in staphylococcal species

Mariko Ikuo, Gentaro Nagano, Yuki Saito, Han Mao, Kazuhisa Sekimizu, Chikara Kaito

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Staphylococcal species acquire antibiotic resistance by incorporating the mobile-genetic element SCCmec. We previously found that SCCmec -encoded psm-mec RNA suppresses exotoxin production as a regulatory RNA, and the psm-mec translation product increases biofilm formation in Staphylococcus aureus. Here, we examined whether the regulatory role of psm-mec on host bacterial virulence properties is conserved among other staphylococcal species, S. epidermidis and S. haemolyticus, both of which are important causes of nosocomial infections. In S. epidermidis, introduction of psm-mec decreased the production of cytolytic toxins called phenol-soluble modulins (PSMs) and increased biofilm formation. Introduction of psm-mec with a stop-codon mutation that did not express PSM-mec protein but did express psm-mec RNA also decreased PSM production, but did not increase biofilm formation. Thus, the psm-mec RNA inhibits PSM production, whereas the PSM-mec protein increases biofilm formation in S. epidermidis. In S. haemolyticus, introduction of psm-mec decreased PSM production, but did not affect biofilm formation. The mutated psm-mec with a stop-codon also caused the same effect. Thus, the psm-mec RNA also inhibits PSM production in S. haemolyticus. These findings suggest that the inhibitory role of psm-mec RNA on exotoxin production is conserved among staphylococcal species, although the stimulating effect of the psm-mec gene on biofilm formation is not conserved.

Original languageEnglish
Article numbere100260
JournalPloS one
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 13 2014
Externally publishedYes

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Interspersed Repetitive Sequences
exotoxins
Exotoxins
Biofilms
phenol
biofilm
RNA
Terminator Codon
stop codon
cross infection
Staphylococcus epidermidis
Microbial Drug Resistance
Cross Infection
staphylococcal delta toxin
interspersed repetitive sequences
antibiotic resistance
translation (genetics)
Virulence
Staphylococcus aureus
Proteins

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Inhibition of exotoxin production by mobile genetic element SCC mec-encoded psm-mec RNA is conserved in staphylococcal species. / Ikuo, Mariko; Nagano, Gentaro; Saito, Yuki; Mao, Han; Sekimizu, Kazuhisa; Kaito, Chikara.

In: PloS one, Vol. 9, No. 6, e100260, 13.06.2014.

Research output: Contribution to journalArticle

Ikuo, Mariko ; Nagano, Gentaro ; Saito, Yuki ; Mao, Han ; Sekimizu, Kazuhisa ; Kaito, Chikara. / Inhibition of exotoxin production by mobile genetic element SCC mec-encoded psm-mec RNA is conserved in staphylococcal species. In: PloS one. 2014 ; Vol. 9, No. 6.
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