Abstract
We have studied the inhibitory effect of chlorophyllin-chitosan (Chl-Chi) complex, an insoluble form of chlorophyllin, on the DNA adduct formation and mutagenesis by a heterocyclic food mutagen-carcinogen, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), in mice carrying the E. coli rpsL gene as a mutagenesis reporter. Upon administration of a diet containing 0.002% or 0.01% Trp-P-2, DNA adducts were formed in various tissues in a dose-dependent manner, with the maximum level observed in the liver. Addition of 3% Chl-Chi to the diet reduced the Trp-P-2 adduct by up to 90%. The rpsL mutant frequencies increased significantly in both the liver and spleen upon administration of a 0.01% Trp-P-2 diet. Addition of Chl-Chi to the diet decreased these induced mutant frequencies to the background level. No harmful effect of Chl-Chi was detected during these experiments. The results show that Chl-Chi may be a candidate chemopreventive agent against the genotoxic action of Trp-P-2, and possibly also other aromatic carcinogens in the diet.
Original language | English |
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Pages (from-to) | 848-853 |
Number of pages | 6 |
Journal | Japanese Journal of Cancer Research |
Volume | 92 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2001 |
Externally published | Yes |
Keywords
- Chemoprevention
- Chitosan
- Chlorophyllin
- Somatic mutation
- Trp-P-2
ASJC Scopus subject areas
- Oncology
- Cancer Research