Inhibition of DNA adduct formation and mutagenic action of 3-amino-1-methyl-5H-pyrido[4,3-b]indole by chlorophyllin-chitosan in rpsL transgenic mice

N. Anzai, T. Taniyama, N. Nakandakari, C. Sugiyama, T. Negishi, H. Hayatsu, K. Negishi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have studied the inhibitory effect of chlorophyllin-chitosan (Chl-Chi) complex, an insoluble form of chlorophyllin, on the DNA adduct formation and mutagenesis by a heterocyclic food mutagen-carcinogen, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), in mice carrying the E. coli rpsL gene as a mutagenesis reporter. Upon administration of a diet containing 0.002% or 0.01% Trp-P-2, DNA adducts were formed in various tissues in a dose-dependent manner, with the maximum level observed in the liver. Addition of 3% Chl-Chi to the diet reduced the Trp-P-2 adduct by up to 90%. The rpsL mutant frequencies increased significantly in both the liver and spleen upon administration of a 0.01% Trp-P-2 diet. Addition of Chl-Chi to the diet decreased these induced mutant frequencies to the background level. No harmful effect of Chl-Chi was detected during these experiments. The results show that Chl-Chi may be a candidate chemopreventive agent against the genotoxic action of Trp-P-2, and possibly also other aromatic carcinogens in the diet.

Original languageEnglish
Pages (from-to)848-853
Number of pages6
JournalJapanese Journal of Cancer Research
Volume92
Issue number8
Publication statusPublished - 2001

Fingerprint

DNA Adducts
Chitosan
Transgenic Mice
Diet
Mutagenesis
Carcinogens
Liver
Mutagens
3-amino-1-methyl-5H-pyrido(4,3-b)indole
chlorophyllin
Spleen
Escherichia coli
Food
Genes

Keywords

  • Chemoprevention
  • Chitosan
  • Chlorophyllin
  • Somatic mutation
  • Trp-P-2

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Anzai, N., Taniyama, T., Nakandakari, N., Sugiyama, C., Negishi, T., Hayatsu, H., & Negishi, K. (2001). Inhibition of DNA adduct formation and mutagenic action of 3-amino-1-methyl-5H-pyrido[4,3-b]indole by chlorophyllin-chitosan in rpsL transgenic mice. Japanese Journal of Cancer Research, 92(8), 848-853.

Inhibition of DNA adduct formation and mutagenic action of 3-amino-1-methyl-5H-pyrido[4,3-b]indole by chlorophyllin-chitosan in rpsL transgenic mice. / Anzai, N.; Taniyama, T.; Nakandakari, N.; Sugiyama, C.; Negishi, T.; Hayatsu, H.; Negishi, K.

In: Japanese Journal of Cancer Research, Vol. 92, No. 8, 2001, p. 848-853.

Research output: Contribution to journalArticle

Anzai, N, Taniyama, T, Nakandakari, N, Sugiyama, C, Negishi, T, Hayatsu, H & Negishi, K 2001, 'Inhibition of DNA adduct formation and mutagenic action of 3-amino-1-methyl-5H-pyrido[4,3-b]indole by chlorophyllin-chitosan in rpsL transgenic mice', Japanese Journal of Cancer Research, vol. 92, no. 8, pp. 848-853.
Anzai, N. ; Taniyama, T. ; Nakandakari, N. ; Sugiyama, C. ; Negishi, T. ; Hayatsu, H. ; Negishi, K. / Inhibition of DNA adduct formation and mutagenic action of 3-amino-1-methyl-5H-pyrido[4,3-b]indole by chlorophyllin-chitosan in rpsL transgenic mice. In: Japanese Journal of Cancer Research. 2001 ; Vol. 92, No. 8. pp. 848-853.
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