Inhibition of DNA adduct formation and mutagenic action of 3-amino-1-methyl-5H-pyrido[4,3-b]indole by chlorophyllin-chitosan in rpsL transgenic mice

Nobuhiro Anzai, Takehiro Taniyama, Namiko Nakandakari, Chitose Sugiyama, Tomoe Negishi, Hikoya Hayatsu, Kazuo Negishi

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We have studied the inhibitory effect of chlorophyllin-chitosan (Chl-Chi) complex, an insoluble form of chlorophyllin, on the DNA adduct formation and mutagenesis by a heterocyclic food mutagen-carcinogen, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), in mice carrying the E. coli rpsL gene as a mutagenesis reporter. Upon administration of a diet containing 0.002% or 0.01% Trp-P-2, DNA adducts were formed in various tissues in a dose-dependent manner, with the maximum level observed in the liver. Addition of 3% Chl-Chi to the diet reduced the Trp-P-2 adduct by up to 90%. The rpsL mutant frequencies increased significantly in both the liver and spleen upon administration of a 0.01% Trp-P-2 diet. Addition of Chl-Chi to the diet decreased these induced mutant frequencies to the background level. No harmful effect of Chl-Chi was detected during these experiments. The results show that Chl-Chi may be a candidate chemopreventive agent against the genotoxic action of Trp-P-2, and possibly also other aromatic carcinogens in the diet.

Original languageEnglish
Pages (from-to)848-853
Number of pages6
JournalJapanese Journal of Cancer Research
Volume92
Issue number8
DOIs
Publication statusPublished - 2001

Keywords

  • Chemoprevention
  • Chitosan
  • Chlorophyllin
  • Somatic mutation
  • Trp-P-2

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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