Abstract
We have studied the inhibitory effect of chlorophyllin-chitosan (Chl-Chi) complex, an insoluble form of chlorophyllin, on the DNA adduct formation and mutagenesis by a heterocyclic food mutagen-carcinogen, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), in mice carrying the E. coli rpsL gene as a mutagenesis reporter. Upon administration of a diet containing 0.002% or 0.01% Trp-P-2, DNA adducts were formed in various tissues in a dose-dependent manner, with the maximum level observed in the liver. Addition of 3% Chl-Chi to the diet reduced the Trp-P-2 adduct by up to 90%. The rpsL mutant frequencies increased significantly in both the liver and spleen upon administration of a 0.01% Trp-P-2 diet. Addition of Chl-Chi to the diet decreased these induced mutant frequencies to the background level. No harmful effect of Chl-Chi was detected during these experiments. The results show that Chl-Chi may be a candidate chemopreventive agent against the genotoxic action of Trp-P-2, and possibly also other aromatic carcinogens in the diet.
| Original language | English |
|---|---|
| Pages (from-to) | 848-853 |
| Number of pages | 6 |
| Journal | Japanese Journal of Cancer Research |
| Volume | 92 |
| Issue number | 8 |
| Publication status | Published - 2001 |
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Keywords
- Chemoprevention
- Chitosan
- Chlorophyllin
- Somatic mutation
- Trp-P-2
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Inhibition of DNA adduct formation and mutagenic action of 3-amino-1-methyl-5H-pyrido[4,3-b]indole by chlorophyllin-chitosan in rpsL transgenic mice. / Anzai, N.; Taniyama, T.; Nakandakari, N.; Sugiyama, C.; Negishi, T.; Hayatsu, H.; Negishi, K.
In: Japanese Journal of Cancer Research, Vol. 92, No. 8, 2001, p. 848-853.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of DNA adduct formation and mutagenic action of 3-amino-1-methyl-5H-pyrido[4,3-b]indole by chlorophyllin-chitosan in rpsL transgenic mice
AU - Anzai, N.
AU - Taniyama, T.
AU - Nakandakari, N.
AU - Sugiyama, C.
AU - Negishi, T.
AU - Hayatsu, H.
AU - Negishi, K.
PY - 2001
Y1 - 2001
N2 - We have studied the inhibitory effect of chlorophyllin-chitosan (Chl-Chi) complex, an insoluble form of chlorophyllin, on the DNA adduct formation and mutagenesis by a heterocyclic food mutagen-carcinogen, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), in mice carrying the E. coli rpsL gene as a mutagenesis reporter. Upon administration of a diet containing 0.002% or 0.01% Trp-P-2, DNA adducts were formed in various tissues in a dose-dependent manner, with the maximum level observed in the liver. Addition of 3% Chl-Chi to the diet reduced the Trp-P-2 adduct by up to 90%. The rpsL mutant frequencies increased significantly in both the liver and spleen upon administration of a 0.01% Trp-P-2 diet. Addition of Chl-Chi to the diet decreased these induced mutant frequencies to the background level. No harmful effect of Chl-Chi was detected during these experiments. The results show that Chl-Chi may be a candidate chemopreventive agent against the genotoxic action of Trp-P-2, and possibly also other aromatic carcinogens in the diet.
AB - We have studied the inhibitory effect of chlorophyllin-chitosan (Chl-Chi) complex, an insoluble form of chlorophyllin, on the DNA adduct formation and mutagenesis by a heterocyclic food mutagen-carcinogen, 3-amino-1-methyl-5H-pyrido[4,3-b]indole (Trp-P-2), in mice carrying the E. coli rpsL gene as a mutagenesis reporter. Upon administration of a diet containing 0.002% or 0.01% Trp-P-2, DNA adducts were formed in various tissues in a dose-dependent manner, with the maximum level observed in the liver. Addition of 3% Chl-Chi to the diet reduced the Trp-P-2 adduct by up to 90%. The rpsL mutant frequencies increased significantly in both the liver and spleen upon administration of a 0.01% Trp-P-2 diet. Addition of Chl-Chi to the diet decreased these induced mutant frequencies to the background level. No harmful effect of Chl-Chi was detected during these experiments. The results show that Chl-Chi may be a candidate chemopreventive agent against the genotoxic action of Trp-P-2, and possibly also other aromatic carcinogens in the diet.
KW - Chemoprevention
KW - Chitosan
KW - Chlorophyllin
KW - Somatic mutation
KW - Trp-P-2
UR - http://www.scopus.com/inward/record.url?scp=0034826667&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034826667&partnerID=8YFLogxK
M3 - Article
C2 - 11509116
AN - SCOPUS:0034826667
VL - 92
SP - 848
EP - 853
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 8
ER -