Inhibition of colony-spreading activity of Staphylococcus aureus by secretion of δ-hemolysin

Yosuke Omae, Kazuhisa Sekimizu, Chikara Kaito

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Abstract

Staphylococcus aureus spreads on the surface of soft agar, a phenomenon we termed "colony spreading." Here, we found that S. aureus culture supernatant inhibited colony spreading. We purified δ-hemolysin (Hld, δ-toxin), a major protein secreted from S. aureus, as a compound that inhibits colony spreading. The culture supernatants of hld-disrupted mutants had 30-fold lower colony-spreading inhibitory activity than those of the parent strain. Furthermore, hld-disrupted mutants had higher colony-spreading ability than the parent strain. These results suggest that S. aureus negatively regulates colony spreading by secreting δ-hemolysin.

Original languageEnglish
Pages (from-to)15570-15579
Number of pages10
JournalJournal of Biological Chemistry
Volume287
Issue number19
DOIs
Publication statusPublished - May 4 2012
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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