TY - JOUR
T1 - Inhibition of colony-spreading activity of Staphylococcus aureus by secretion of δ-hemolysin
AU - Omae, Yosuke
AU - Sekimizu, Kazuhisa
AU - Kaito, Chikara
PY - 2012/5/4
Y1 - 2012/5/4
N2 - Staphylococcus aureus spreads on the surface of soft agar, a phenomenon we termed "colony spreading." Here, we found that S. aureus culture supernatant inhibited colony spreading. We purified δ-hemolysin (Hld, δ-toxin), a major protein secreted from S. aureus, as a compound that inhibits colony spreading. The culture supernatants of hld-disrupted mutants had 30-fold lower colony-spreading inhibitory activity than those of the parent strain. Furthermore, hld-disrupted mutants had higher colony-spreading ability than the parent strain. These results suggest that S. aureus negatively regulates colony spreading by secreting δ-hemolysin.
AB - Staphylococcus aureus spreads on the surface of soft agar, a phenomenon we termed "colony spreading." Here, we found that S. aureus culture supernatant inhibited colony spreading. We purified δ-hemolysin (Hld, δ-toxin), a major protein secreted from S. aureus, as a compound that inhibits colony spreading. The culture supernatants of hld-disrupted mutants had 30-fold lower colony-spreading inhibitory activity than those of the parent strain. Furthermore, hld-disrupted mutants had higher colony-spreading ability than the parent strain. These results suggest that S. aureus negatively regulates colony spreading by secreting δ-hemolysin.
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U2 - 10.1074/jbc.M112.357848
DO - 10.1074/jbc.M112.357848
M3 - Article
C2 - 22411996
AN - SCOPUS:84860851423
SN - 0021-9258
VL - 287
SP - 15570
EP - 15579
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 19
ER -