Inhibition of biosynthesis of metalloprotease of Aeromonas sobria by sodium chloride in the medium

Eizo Takahashi, Hidetomo Kobayashi, Hiroyasu Yamanaka, Gopinath Balakrish Nair, Yoshifumi Takeda, Sakae Arimoto, Tomoe Negishi, Keinosuke Okamoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present authors have previously shown that the serine protease activity of Aeromonas sobria is markedly decreased when A. sobria is cultured in medium containing 3.0% sodium chloride (NaCl, concentration almost equivalent to sea water salinity), and that this occurs because, although the synthesis of ASP is not disturbed by the salt in the medium, the maturation pathway of serine protease of A. sobria (ASP) does not proceed successfully in such a medium. In this study, the effect of salt in the medium on the production of metalloprotease by A. sobria (AMP) was examined. A. sobria produced AMP in the milieu when the bacteria were cultured in medium containing (NaCl) at a concentration of 0.5%. However, AMP was not produced when the bacteria were cultured in salty medium containing 1.5% or more NaCl. To examine how NaCl reduces the production of metalloprotease by A. sobria, the amount of amp mRNA in the cell was measured and it was found that this decreased in proportion to the concentration of NaCl in the medium. The mRNA of amp was not detected in cells cultured in medium containing 1.5% or more NaCl. This means that the transcription of amp is inhibited in salty condition. As described, NaCl in the medium disturbs the maturation pathway of ASP. The mode of action whereby NaCl suppresses AMP activity in A. sobria differs from the mechanism for suppressing ASP activity.

Original languageEnglish
Pages (from-to)60-65
Number of pages6
JournalMicrobiology and Immunology
Volume55
Issue number1
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Aeromonas
Metalloproteases
Adenosine Monophosphate
Sodium Chloride
Serine Proteases
Salts
Bacteria
Messenger RNA
Salinity
Seawater
Cultured Cells

Keywords

  • Aeromonas sobria
  • Metalloprotease
  • Sodium chloride
  • Transcription

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology

Cite this

Inhibition of biosynthesis of metalloprotease of Aeromonas sobria by sodium chloride in the medium. / Takahashi, Eizo; Kobayashi, Hidetomo; Yamanaka, Hiroyasu; Nair, Gopinath Balakrish; Takeda, Yoshifumi; Arimoto, Sakae; Negishi, Tomoe; Okamoto, Keinosuke.

In: Microbiology and Immunology, Vol. 55, No. 1, 01.2011, p. 60-65.

Research output: Contribution to journalArticle

Takahashi, Eizo ; Kobayashi, Hidetomo ; Yamanaka, Hiroyasu ; Nair, Gopinath Balakrish ; Takeda, Yoshifumi ; Arimoto, Sakae ; Negishi, Tomoe ; Okamoto, Keinosuke. / Inhibition of biosynthesis of metalloprotease of Aeromonas sobria by sodium chloride in the medium. In: Microbiology and Immunology. 2011 ; Vol. 55, No. 1. pp. 60-65.
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