Inhibition of auxin-induced ethylene production in cucumber fruit discs by carbon dioxide

Francis M. Mathooko, Yasutaka Kubo, Akitsugu Inaba, Reinosuke Nakamura

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The induction of ethylene biosynthesis by indole-3-acetic acid (IAA) and its regulation by CO2 was examined in cucumber (Cucumis sativus L., var. Sharp-1) fruit discs. Treatment of discs with IAA significantly stimulated ethylene production and the extent depended on IAA concentration upto 1 mM. The increase in IAA-induced ethylene production was accompanied by increase in the activities of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase and ACC oxidase (also known as ethylene-forming enzyme) and a massive accumulation of ACC and 1-(malonylamino)cyclopropane-1-carboxylic acid (MACC). The induction of ethylene production by IAA was inhibited by treatment with CO2. The extent of inhibition was dependent upon the concentration of CO2. Carbon dioxide inhibited IAA-induced ethylene biosynthesis by suppressing ACC synthase and ACC oxidase activities. Additionally, CO2 inhibited ACC conjugation into MACC. The results indicate that inhibition of ethylene production by CO2 in cucumber fruit discs following IAA treatment is due to inhibition of both ACC synthesis and ACC oxidation.

Original languageEnglish
Pages (from-to)313-325
Number of pages13
JournalPostharvest Biology and Technology
Volume3
Issue number4
DOIs
Publication statusPublished - 1993

Fingerprint

Cucumis sativus
Indoleacetic Acids
1-aminocyclopropane-1-carboxylic acid
Carbon Dioxide
ethylene production
indole acetic acid
cucumbers
auxins
Fruit
carbon dioxide
fruits
carboxylic acids
Oxidoreductases
1-aminocyclopropane-1-carboxylate synthase
aminocyclopropanecarboxylate oxidase
ethylene
acid treatment
indoleacetic acid
oxidation
synthesis

Keywords

  • Auxin
  • CO
  • Cucumber
  • Cucumis sativus
  • Ethylene

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Horticulture

Cite this

Inhibition of auxin-induced ethylene production in cucumber fruit discs by carbon dioxide. / Mathooko, Francis M.; Kubo, Yasutaka; Inaba, Akitsugu; Nakamura, Reinosuke.

In: Postharvest Biology and Technology, Vol. 3, No. 4, 1993, p. 313-325.

Research output: Contribution to journalArticle

Mathooko, Francis M. ; Kubo, Yasutaka ; Inaba, Akitsugu ; Nakamura, Reinosuke. / Inhibition of auxin-induced ethylene production in cucumber fruit discs by carbon dioxide. In: Postharvest Biology and Technology. 1993 ; Vol. 3, No. 4. pp. 313-325.
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