Abstract
A pathogenic fungus of pea, Mycosphaerella pinodes, secretes a so-called "suppressor" in its pycnospore germination fluid. The suppressor blocks the defense responses and induces local susceptibility (accessibility) in pea plants to agents that are not pathogenic in pea. The suppressor nonspecifically inhibits the ATPase activity in plasma membranes prepared from pea, soybean, kidney bean, cowpea and barley plants. However, cytochemical studies by electron microscopy indicate that the suppressor specifically inhibits the ATPase in pea cell membranes, but not in those of four other plant species tested. That is, the specificity of the suppressor appears at the cell and/or tissue level, but is not evident in vitro. Furthermore, the inhibitory effect of the suppressor is temporary because the ATPase activity recovers 9 h after the treatment. A similar effect was observed after inoculation with M. pinodes but not with a nonpathogen of pea, M. ligulicola. The role of the suppressor in host-parasite specificity is discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 1067-1075 |
| Number of pages | 9 |
| Journal | Plant and Cell Physiology |
| Volume | 32 |
| Issue number | 7 |
| Publication status | Published - Oct 1991 |
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Keywords
- ATPase
- Mycosphaerella pinodes
- Pisum sativum L.
- Plasma membrane
- Suppressor
- TEM
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Ecology
- Cell Biology
- Physiology
- Plant Science
Cite this
Inhibition of ATPase activity in pea plasma membranes in situ by a suppressor from a pea pathogen, mycosphaerella pinodes. / Shiraishi, Tomonori; Araki, Miwa; Yoshioka, Hirofumi; Kobayashi, Issei; Yamada, Tetsuji; Ichinose, Yuki; Kunoh, Hitoshi; Oku, Hachiro.
In: Plant and Cell Physiology, Vol. 32, No. 7, 10.1991, p. 1067-1075.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Inhibition of ATPase activity in pea plasma membranes in situ by a suppressor from a pea pathogen, mycosphaerella pinodes
AU - Shiraishi, Tomonori
AU - Araki, Miwa
AU - Yoshioka, Hirofumi
AU - Kobayashi, Issei
AU - Yamada, Tetsuji
AU - Ichinose, Yuki
AU - Kunoh, Hitoshi
AU - Oku, Hachiro
PY - 1991/10
Y1 - 1991/10
N2 - A pathogenic fungus of pea, Mycosphaerella pinodes, secretes a so-called "suppressor" in its pycnospore germination fluid. The suppressor blocks the defense responses and induces local susceptibility (accessibility) in pea plants to agents that are not pathogenic in pea. The suppressor nonspecifically inhibits the ATPase activity in plasma membranes prepared from pea, soybean, kidney bean, cowpea and barley plants. However, cytochemical studies by electron microscopy indicate that the suppressor specifically inhibits the ATPase in pea cell membranes, but not in those of four other plant species tested. That is, the specificity of the suppressor appears at the cell and/or tissue level, but is not evident in vitro. Furthermore, the inhibitory effect of the suppressor is temporary because the ATPase activity recovers 9 h after the treatment. A similar effect was observed after inoculation with M. pinodes but not with a nonpathogen of pea, M. ligulicola. The role of the suppressor in host-parasite specificity is discussed.
AB - A pathogenic fungus of pea, Mycosphaerella pinodes, secretes a so-called "suppressor" in its pycnospore germination fluid. The suppressor blocks the defense responses and induces local susceptibility (accessibility) in pea plants to agents that are not pathogenic in pea. The suppressor nonspecifically inhibits the ATPase activity in plasma membranes prepared from pea, soybean, kidney bean, cowpea and barley plants. However, cytochemical studies by electron microscopy indicate that the suppressor specifically inhibits the ATPase in pea cell membranes, but not in those of four other plant species tested. That is, the specificity of the suppressor appears at the cell and/or tissue level, but is not evident in vitro. Furthermore, the inhibitory effect of the suppressor is temporary because the ATPase activity recovers 9 h after the treatment. A similar effect was observed after inoculation with M. pinodes but not with a nonpathogen of pea, M. ligulicola. The role of the suppressor in host-parasite specificity is discussed.
KW - ATPase
KW - Mycosphaerella pinodes
KW - Pisum sativum L.
KW - Plasma membrane
KW - Suppressor
KW - TEM
UR - http://www.scopus.com/inward/record.url?scp=0347130204&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347130204&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0347130204
VL - 32
SP - 1067
EP - 1075
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 7
ER -