Inhibition of ATPase activity in pea plasma membranes in situ by a suppressor from a pea pathogen, mycosphaerella pinodes

Tomonori Shiraishi, Miwa Araki, Hirofumi Yoshioka, Issei Kobayashi, Tetsuji Yamada, Yuki Ichinose, Hitoshi Kunoh, Hachiro Oku

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

A pathogenic fungus of pea, Mycosphaerella pinodes, secretes a so-called "suppressor" in its pycnospore germination fluid. The suppressor blocks the defense responses and induces local susceptibility (accessibility) in pea plants to agents that are not pathogenic in pea. The suppressor nonspecifically inhibits the ATPase activity in plasma membranes prepared from pea, soybean, kidney bean, cowpea and barley plants. However, cytochemical studies by electron microscopy indicate that the suppressor specifically inhibits the ATPase in pea cell membranes, but not in those of four other plant species tested. That is, the specificity of the suppressor appears at the cell and/or tissue level, but is not evident in vitro. Furthermore, the inhibitory effect of the suppressor is temporary because the ATPase activity recovers 9 h after the treatment. A similar effect was observed after inoculation with M. pinodes but not with a nonpathogen of pea, M. ligulicola. The role of the suppressor in host-parasite specificity is discussed.

Original languageEnglish
Pages (from-to)1067-1075
Number of pages9
JournalPlant and Cell Physiology
Volume32
Issue number7
Publication statusPublished - Oct 1991

Fingerprint

Mycosphaerella pinodes
Plasma Membrane
ATP Synthase
Peas
Pathogens
Cell membranes
adenosinetriphosphatase
Adenosine Triphosphatases
peas
plasma membrane
pathogen
Cell Membrane
membrane
plasma
pathogens
electron microscopy
barley
accessibility
inoculation
soybean

Keywords

  • ATPase
  • Mycosphaerella pinodes
  • Pisum sativum L.
  • Plasma membrane
  • Suppressor
  • TEM

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Shiraishi, T., Araki, M., Yoshioka, H., Kobayashi, I., Yamada, T., Ichinose, Y., ... Oku, H. (1991). Inhibition of ATPase activity in pea plasma membranes in situ by a suppressor from a pea pathogen, mycosphaerella pinodes. Plant and Cell Physiology, 32(7), 1067-1075.

Inhibition of ATPase activity in pea plasma membranes in situ by a suppressor from a pea pathogen, mycosphaerella pinodes. / Shiraishi, Tomonori; Araki, Miwa; Yoshioka, Hirofumi; Kobayashi, Issei; Yamada, Tetsuji; Ichinose, Yuki; Kunoh, Hitoshi; Oku, Hachiro.

In: Plant and Cell Physiology, Vol. 32, No. 7, 10.1991, p. 1067-1075.

Research output: Contribution to journalArticle

Shiraishi, T, Araki, M, Yoshioka, H, Kobayashi, I, Yamada, T, Ichinose, Y, Kunoh, H & Oku, H 1991, 'Inhibition of ATPase activity in pea plasma membranes in situ by a suppressor from a pea pathogen, mycosphaerella pinodes', Plant and Cell Physiology, vol. 32, no. 7, pp. 1067-1075.
Shiraishi, Tomonori ; Araki, Miwa ; Yoshioka, Hirofumi ; Kobayashi, Issei ; Yamada, Tetsuji ; Ichinose, Yuki ; Kunoh, Hitoshi ; Oku, Hachiro. / Inhibition of ATPase activity in pea plasma membranes in situ by a suppressor from a pea pathogen, mycosphaerella pinodes. In: Plant and Cell Physiology. 1991 ; Vol. 32, No. 7. pp. 1067-1075.
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