Abstract
The effect of chlorophyllin on 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx)-mediated DNA-adduct formation in Drosophila was studied. Third-instar larvae of Drosophila were fed MeIQx at 1 mg/6.5 g-feed/bottle, with or without chlorophyllin (100-300 mg). After a 6 h feeding exposure to MeIQx, the larvae were divided into 2 groups. The first group was examined for covalent DNA adducts by 32P-postlabeling assay. The second group was assayed for DNA damage by allowing the larvae to develop to adults and measuring the male/female ratio (males, DNA repair-deficient; females, DNA repair-proficient). The 32P-postlabeling results indicated a significant decrease in DNA adduct levels in larvae treated with MeIQx and 300 mg chlorophyllin (1.7±0.7 adducts/107 nucleotides) as compared with MeIQx-treated larvae (6.5±2.1 adducts/107 nucleotides). The results on male/female sex ratios also indicated a chlorophyllin-induced decrease in DNA damage by exposure to MeIQx. The suppressive effect of chlorophyllin on the genotoxic actions of a polycyclic mutagen, MeIQx, may be a result of complex formation between chlorophyllin and the mutagen.
| Original language | English |
|---|---|
| Pages (from-to) | 325-328 |
| Number of pages | 4 |
| Journal | Japanese Journal of Cancer Research |
| Volume | 87 |
| Issue number | 4 |
| Publication status | Published - Apr 1996 |
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Keywords
- P-Postlabeling of Drosophila DNA
- Chlorophyllin
- MeIQx
- MeIQx-DNA adduct
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Inhibition of 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline-mediated DNA-adduct formation by chlorophyllin in Drosophila. / Sugiyama, Chitose; Shinoda, Aki; Hayatsu, Hikoya; Negishi, Tomoe.
In: Japanese Journal of Cancer Research, Vol. 87, No. 4, 04.1996, p. 325-328.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibition of 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline-mediated DNA-adduct formation by chlorophyllin in Drosophila
AU - Sugiyama, Chitose
AU - Shinoda, Aki
AU - Hayatsu, Hikoya
AU - Negishi, Tomoe
PY - 1996/4
Y1 - 1996/4
N2 - The effect of chlorophyllin on 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx)-mediated DNA-adduct formation in Drosophila was studied. Third-instar larvae of Drosophila were fed MeIQx at 1 mg/6.5 g-feed/bottle, with or without chlorophyllin (100-300 mg). After a 6 h feeding exposure to MeIQx, the larvae were divided into 2 groups. The first group was examined for covalent DNA adducts by 32P-postlabeling assay. The second group was assayed for DNA damage by allowing the larvae to develop to adults and measuring the male/female ratio (males, DNA repair-deficient; females, DNA repair-proficient). The 32P-postlabeling results indicated a significant decrease in DNA adduct levels in larvae treated with MeIQx and 300 mg chlorophyllin (1.7±0.7 adducts/107 nucleotides) as compared with MeIQx-treated larvae (6.5±2.1 adducts/107 nucleotides). The results on male/female sex ratios also indicated a chlorophyllin-induced decrease in DNA damage by exposure to MeIQx. The suppressive effect of chlorophyllin on the genotoxic actions of a polycyclic mutagen, MeIQx, may be a result of complex formation between chlorophyllin and the mutagen.
AB - The effect of chlorophyllin on 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx)-mediated DNA-adduct formation in Drosophila was studied. Third-instar larvae of Drosophila were fed MeIQx at 1 mg/6.5 g-feed/bottle, with or without chlorophyllin (100-300 mg). After a 6 h feeding exposure to MeIQx, the larvae were divided into 2 groups. The first group was examined for covalent DNA adducts by 32P-postlabeling assay. The second group was assayed for DNA damage by allowing the larvae to develop to adults and measuring the male/female ratio (males, DNA repair-deficient; females, DNA repair-proficient). The 32P-postlabeling results indicated a significant decrease in DNA adduct levels in larvae treated with MeIQx and 300 mg chlorophyllin (1.7±0.7 adducts/107 nucleotides) as compared with MeIQx-treated larvae (6.5±2.1 adducts/107 nucleotides). The results on male/female sex ratios also indicated a chlorophyllin-induced decrease in DNA damage by exposure to MeIQx. The suppressive effect of chlorophyllin on the genotoxic actions of a polycyclic mutagen, MeIQx, may be a result of complex formation between chlorophyllin and the mutagen.
KW - P-Postlabeling of Drosophila DNA
KW - Chlorophyllin
KW - MeIQx
KW - MeIQx-DNA adduct
UR - http://www.scopus.com/inward/record.url?scp=0029871426&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029871426&partnerID=8YFLogxK
M3 - Article
VL - 87
SP - 325
EP - 328
JO - Cancer Science
JF - Cancer Science
SN - 1347-9032
IS - 4
ER -