Inhibition of 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline-mediated DNA-adduct formation by chlorophyllin in Drosophila

Chitose Sugiyama, Aki Shinoda, Hikoya Hayatsu, Tomoe Negishi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effect of chlorophyllin on 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx)-mediated DNA-adduct formation in Drosophila was studied. Third-instar larvae of Drosophila were fed MeIQx at 1 mg/6.5 g-feed/bottle, with or without chlorophyllin (100-300 mg). After a 6 h feeding exposure to MeIQx, the larvae were divided into 2 groups. The first group was examined for covalent DNA adducts by 32P-postlabeling assay. The second group was assayed for DNA damage by allowing the larvae to develop to adults and measuring the male/female ratio (males, DNA repair-deficient; females, DNA repair-proficient). The 32P-postlabeling results indicated a significant decrease in DNA adduct levels in larvae treated with MeIQx and 300 mg chlorophyllin (1.7±0.7 adducts/107 nucleotides) as compared with MeIQx-treated larvae (6.5±2.1 adducts/107 nucleotides). The results on male/female sex ratios also indicated a chlorophyllin-induced decrease in DNA damage by exposure to MeIQx. The suppressive effect of chlorophyllin on the genotoxic actions of a polycyclic mutagen, MeIQx, may be a result of complex formation between chlorophyllin and the mutagen.

Original languageEnglish
Pages (from-to)325-328
Number of pages4
JournalJapanese Journal of Cancer Research
Volume87
Issue number4
DOIs
Publication statusPublished - Apr 1996

Keywords

  • Chlorophyllin
  • MeIQx
  • MeIQx-DNA adduct
  • P-Postlabeling of Drosophila DNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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