Inhibition of 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline-mediated DNA-adduct formation by chlorophyllin in Drosophila

Chitose Sugiyama, Aki Shinoda, Hikoya Hayatsu, Tomoe Negishi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The effect of chlorophyllin on 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (MeIQx)-mediated DNA-adduct formation in Drosophila was studied. Third-instar larvae of Drosophila were fed MeIQx at 1 mg/6.5 g-feed/bottle, with or without chlorophyllin (100-300 mg). After a 6 h feeding exposure to MeIQx, the larvae were divided into 2 groups. The first group was examined for covalent DNA adducts by 32P-postlabeling assay. The second group was assayed for DNA damage by allowing the larvae to develop to adults and measuring the male/female ratio (males, DNA repair-deficient; females, DNA repair-proficient). The 32P-postlabeling results indicated a significant decrease in DNA adduct levels in larvae treated with MeIQx and 300 mg chlorophyllin (1.7±0.7 adducts/107 nucleotides) as compared with MeIQx-treated larvae (6.5±2.1 adducts/107 nucleotides). The results on male/female sex ratios also indicated a chlorophyllin-induced decrease in DNA damage by exposure to MeIQx. The suppressive effect of chlorophyllin on the genotoxic actions of a polycyclic mutagen, MeIQx, may be a result of complex formation between chlorophyllin and the mutagen.

Original languageEnglish
Pages (from-to)325-328
Number of pages4
JournalJapanese Journal of Cancer Research
Volume87
Issue number4
Publication statusPublished - Apr 1996

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2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline
DNA Adducts
Drosophila
Larva
Mutagens
DNA Damage
Nucleotides
DNA Repair-Deficiency Disorders
Sex Ratio
DNA Repair
chlorophyllin

Keywords

  • P-Postlabeling of Drosophila DNA
  • Chlorophyllin
  • MeIQx
  • MeIQx-DNA adduct

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Inhibition of 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline-mediated DNA-adduct formation by chlorophyllin in Drosophila. / Sugiyama, Chitose; Shinoda, Aki; Hayatsu, Hikoya; Negishi, Tomoe.

In: Japanese Journal of Cancer Research, Vol. 87, No. 4, 04.1996, p. 325-328.

Research output: Contribution to journalArticle

Sugiyama, Chitose ; Shinoda, Aki ; Hayatsu, Hikoya ; Negishi, Tomoe. / Inhibition of 2-Amino-3,8-dimethylimidazo[4,5-f]quinoxaline-mediated DNA-adduct formation by chlorophyllin in Drosophila. In: Japanese Journal of Cancer Research. 1996 ; Vol. 87, No. 4. pp. 325-328.
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