Inhibition by Zn2+ of A-form monoamine oxidase in monkey brain mitochondria

Toru Egashira, Fusako Takayama, Kumiko Sakai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The effects of ZnSO4 on mitochondrial monoamine oxidase (MAO) activity in monkey brain were compared with those in rat and rabbit, in vitro. After preincubation at 25 °C for 20 min with 1 μM ZnSO4, MAO-A activity in monkey brain was about 50% using serotonin (5-HT) as a substrate, and the inhibition was proportional to the concentration of ZnSO4. However, ZnSO4 had no effect on MAO-B activity in monkey brain using β-phenylethylamine (β-PEA) as a substrate. The inhibition by ZnSO4 of MAO-A activity was competitive and reversible. CdSO4 also inhibits MAO-A, but not MAO-B in monkey brain mitochondria. ZnSO4 did not inhibit either MAO-A or MAO-B activity in rat and rabbit brain mitochondria. These results indicate that the inhibiting action of Zn2+ differs depending on animal species. In monkey brain mitochondria, MAO-A was highly sensitive to Zn2+ and MAO-B was less sensitive. These results also suggest that Zn2+ may regulate the level of catecholamine content in monkey brain.

Original languageEnglish
Pages (from-to)239-245
Number of pages7
JournalJournal Pharmacological Sciences
Volume91
Issue number3
DOIs
Publication statusPublished - Mar 1 2003

Keywords

  • A-form monoamine oxidase
  • B-form monoamine oxidase
  • Monkey brain
  • Zn,

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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