Inhibition by 2-methoxy-4-ethylphenol of Ca2+ influx through acquired and native N-methyl- D-aspartate-receptor channels

Ryo Fukumori, Noritaka Nakamichi, Takeshi Takarada, Yuki Kambe, Nobuyuki Matsushima, Nobuaki Moriguchi, Yukio Yoneda

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Pharmacological properties were evaluated for the antidiarrheic wood creosote ingredient 2-methoxy-4-ethylphenol (2M4EP), which was shown to be protective against neurotoxicity of N -methyl- D -aspartate (NMDA), to modulate Ca2+ influx across acquired and native NMDA re-ceptor (NMDAR) channels. NMDA markedly increased intracellular free Ca2+ levels in HEK293 cells transfected with the expression vector of either NR2A or NR2B subunit together with the essential NR1 subunit vector. Further addition of dizocilpine inhibited the increase by NMDA in intracellular Ca2+ levels in both types of acquired NMDAR channels, while 2M4EP and the NR2B-subunit-selective antagonist ifenprodil were more effective in inhibiting the increase by NMDA in HEK293 cells expressing NR1/NR2B subunits than in those with NR1/NR2A subunits. 2M4EP significantly prevented the increased intracellular Ca2+ levels by NMDA in cultured rat hippocam-pal neurons. Brief exposure to NMDA led to a drastic decrease in cellular viability 24 h later in cultured hippocampal neurons, while 2M4EP significantly prevented the loss of cellular vitality by NMDA. Similarly, 2M4EP more efficiently protected HEK293 cells with NR1/NR2B subunits than those with NR1/NR2A subunits. These results suggest that 2M4EP may protect neurons from excitotoxicity through inhibition of Ca2+ influx across NMDAR channels composed of NR1/ NR2B, rather than NR1/NR2A, subunits.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalJournal of Pharmacological Sciences
Volume112
Issue number3
DOIs
Publication statusPublished - Mar 19 2010
Externally publishedYes

Keywords

  • Acquired channel
  • Antidiarrheic
  • Hippocampal neuron
  • N-methyl-D-aspartate receptor

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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