Inhalation of 10% carbon dioxide rapidly terminates Scn1a mutation-related hyperthermia-induced seizures

Iori Ohmori, Keiichiro Hayashi, Haijiao Wang, Mamoru Oouchida, Naohiro Fujita, Takushi Inoue, Hiroyuki Michiue, Tei-ichi Nishiki, Hideki Matsui

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The aim of this study was to assess the anticonvulsant effect of carbon dioxide (CO2) on Scn1a mutation-related febrile seizures. We examined physiological changes in the blood gas levels after the induction of hyperthermia-induced seizures (HISs), which were associated with the Scn1a missense mutation. We determined the efficacy of inhalation of 5% or 10% CO2 to treat HISs. HISs were evoked in Scn1a mutant and wild-type (WT) rats by hot water baths. To determine the anticonvulsant effect of CO2 inhalation, rats were placed in a chamber filled with air or mixed gas containing 5% CO2 or 10% CO2 for 3min, immediately after the induction of HISs. We also analyzed the blood gas levels at the end of inhalation of CO2. Hot water bathing induced a significant reduction in the partial pressure of CO2 (pCO2) and respiratory alkalosis in the WT and Scn1a mutant rats. HISs were evoked in 100% of the Scn1a mutant rats within 5min, but in none of the WT rats. The Scn1a mutant rats demonstrated a higher HISs susceptibility associated with respiratory alkalosis than the WT rats. Inhalation of 10% CO2 shortened the seizure duration from 62.6±12.1s to 15.5±1.0s. Blood gas analysis after the inhalation of 10% CO2 demonstrated an elevated pCO2 level and respiratory acidosis. Inhalation of 10% CO2 demonstrated a potent and fast-acting anticonvulsant effect against HISs.

Original languageEnglish
Pages (from-to)220-224
Number of pages5
JournalEpilepsy Research
Volume105
Issue number1-2
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Induced Hyperthermia
Carbon Dioxide
Inhalation
Seizures
Mutation
Respiratory Alkalosis
Anticonvulsants
Gases
Respiratory Acidosis
Febrile Seizures
Blood Gas Analysis
Water
Partial Pressure
Missense Mutation
Baths
Air
Pressure

Keywords

  • CO
  • Dravet syndrome
  • Febrile seizure
  • GEFS+
  • SCN1A

ASJC Scopus subject areas

  • Clinical Neurology
  • Neurology

Cite this

Inhalation of 10% carbon dioxide rapidly terminates Scn1a mutation-related hyperthermia-induced seizures. / Ohmori, Iori; Hayashi, Keiichiro; Wang, Haijiao; Oouchida, Mamoru; Fujita, Naohiro; Inoue, Takushi; Michiue, Hiroyuki; Nishiki, Tei-ichi; Matsui, Hideki.

In: Epilepsy Research, Vol. 105, No. 1-2, 07.2013, p. 220-224.

Research output: Contribution to journalArticle

Ohmori, Iori ; Hayashi, Keiichiro ; Wang, Haijiao ; Oouchida, Mamoru ; Fujita, Naohiro ; Inoue, Takushi ; Michiue, Hiroyuki ; Nishiki, Tei-ichi ; Matsui, Hideki. / Inhalation of 10% carbon dioxide rapidly terminates Scn1a mutation-related hyperthermia-induced seizures. In: Epilepsy Research. 2013 ; Vol. 105, No. 1-2. pp. 220-224.
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