Infusion of exogenous tumor necrosis factor dose dependently alters the length of the luteal phase in cattle: Differential responses to treatment with indomethacin and L-NAME, a nitric oxide synthase inhibitor

Dariusz J. Skarzynski, Izabela Woclawek-Potocka, Anna Korzekwa, Mamadou M. Bah, Katarzyna Piotrowska, Beata Barszczewska, Kiyoshi Okuda

Research output: Contribution to journalArticle

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Abstract

We examined whether prostaglandins (PGs) and nitric oxide (NO) mediate tumor necrosis factor (TNF) actions in the estrus cycle. On Day 14 of the cycle, the following solutions were infused into the aorta abdominalis of a total of 51 heifers (Experiments 1 and 2): saline; 1 or 10 μg of TNF; 480 mg indomethacin (INDO), an inhibitor of prostaglandin H synthase; 800 mg L-NAME, an inhibitor of NO synthase; and TNF (1 or 10 μg) in combination with INDO or L-NAME. TNF at 1 μg infused directly into aorta abdominalis increased the level of PGF and decreased the level of progesterone (P4) in the peripheral blood and shortened the estrus cycle. The high TNF dose stimulated P4 and PGE2 and prolonged the corpus luteum (CL) lifespan. INDO blocked the effects of both TNF doses on the CL lifespan and hormone output. L-NAME completely blocked the effects of the luteolytic TNF dose, whereas the effects of the luteotropic TNF dose were not inhibited. In Experiment 3 (Day 14), saline or different TNF doses were infused into the jugular vein (n = 9) or into the uterine lumen (n = 18). The CL lifespans of the different groups were not different when TNF was infused into the jugular vein. Although high TNF doses (1 and 10 μg) infused into the uterine lumen prolonged the CL lifespan, low doses (0.01 and 0.1 μg) induced premature luteolysis. We suggest that the actions of exogenous TNF on the CL lifespan depend on PG synthesis stimulated by TNF in the uterus. TNF at low concentrations initiates a positive cascade between uterine PGF and various luteolytic factors, including NO, to complete premature luteolysis. PGE2 is a good candidate mediator of the luteotropic actions of exogenous TNF action.

Original languageEnglish
Pages (from-to)619-627
Number of pages9
JournalBiology of Reproduction
Volume76
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Luteal Phase
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase
Indomethacin
Tumor Necrosis Factor-alpha
Corpus Luteum
Luteolysis
Jugular Veins
Estrus
Dinoprostone
Prostaglandins
Corpus Luteum Hormones
Aorta
Nitric Oxide
Dinoprost
Prostaglandins F
Prostaglandin-Endoperoxide Synthases
Uterus
Progesterone

Keywords

  • Corpus luteum function
  • Cytokine
  • Estrus cycle
  • Necrosis factor
  • Ovulatory cycle
  • Progesterone
  • Prostaglandins
  • Tumor
  • Uterus

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Infusion of exogenous tumor necrosis factor dose dependently alters the length of the luteal phase in cattle : Differential responses to treatment with indomethacin and L-NAME, a nitric oxide synthase inhibitor. / Skarzynski, Dariusz J.; Woclawek-Potocka, Izabela; Korzekwa, Anna; Bah, Mamadou M.; Piotrowska, Katarzyna; Barszczewska, Beata; Okuda, Kiyoshi.

In: Biology of Reproduction, Vol. 76, No. 4, 04.2007, p. 619-627.

Research output: Contribution to journalArticle

Skarzynski, Dariusz J. ; Woclawek-Potocka, Izabela ; Korzekwa, Anna ; Bah, Mamadou M. ; Piotrowska, Katarzyna ; Barszczewska, Beata ; Okuda, Kiyoshi. / Infusion of exogenous tumor necrosis factor dose dependently alters the length of the luteal phase in cattle : Differential responses to treatment with indomethacin and L-NAME, a nitric oxide synthase inhibitor. In: Biology of Reproduction. 2007 ; Vol. 76, No. 4. pp. 619-627.
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