Infrequent existence of simian virus 40 large T antigen DNA in malignant mesothelioma in Japan

Keisuke Aoe, Akio Hiraki, Tomoyuki Murakami, Shinichi Toyooka, Narayan Shivapurkar, Adi F. Gazdar, Naoko Sueoka, Koji Taguchi, Toshiaki Kamei, Hiroyasu Takeyama, Kazuro Sugi, Takumi Kishimoto

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Malignant mesothelioma is the most common primary pleural neoplasm. Association of simian virus 40 (SV40) with malignant mesothelioma has been reported, suggesting that SV40 plays an important role in the origin of a subset of these tumors. However, significant geographic variation is present as to how often this association occurs. As no study concerning SV40 in malignant mesothelioma has been reported from Japan, we examined the frequency of SV40 infection in Japanese malignant mesothelioma cases. In pleural malignant mesothelioma tissue from 35 patients in Japan, we sought the presence of SV40 large T antigen DNA using real-time polymerase chain reaction (PCR), as well as expression of the viral protein using immunohistological methods. Real-time PCR demonstrated that two of 35 mesotheliomas contained DNA sequences encoding portions of SV40 large T antigen. None of the 35 malignant mesothelioma specimens showed immunoreactivity for SV40 large T antigen. SV40 infection does not appear to have a major role in the development of malignant mesothelioma in Japan.

Original languageEnglish
Pages (from-to)292-295
Number of pages4
JournalCancer Science
Volume97
Issue number4
DOIs
Publication statusPublished - Apr 2006

Fingerprint

Simian virus 40
Viral Tumor Antigens
Japan
Virus Diseases
Pleural Neoplasms
Real-Time Polymerase Chain Reaction
Malignant Mesothelioma
T-DNA
Mesothelioma
Viral Proteins

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Aoe, K., Hiraki, A., Murakami, T., Toyooka, S., Shivapurkar, N., Gazdar, A. F., ... Kishimoto, T. (2006). Infrequent existence of simian virus 40 large T antigen DNA in malignant mesothelioma in Japan. Cancer Science, 97(4), 292-295. https://doi.org/10.1111/j.1349-7006.2006.00171.x

Infrequent existence of simian virus 40 large T antigen DNA in malignant mesothelioma in Japan. / Aoe, Keisuke; Hiraki, Akio; Murakami, Tomoyuki; Toyooka, Shinichi; Shivapurkar, Narayan; Gazdar, Adi F.; Sueoka, Naoko; Taguchi, Koji; Kamei, Toshiaki; Takeyama, Hiroyasu; Sugi, Kazuro; Kishimoto, Takumi.

In: Cancer Science, Vol. 97, No. 4, 04.2006, p. 292-295.

Research output: Contribution to journalArticle

Aoe, K, Hiraki, A, Murakami, T, Toyooka, S, Shivapurkar, N, Gazdar, AF, Sueoka, N, Taguchi, K, Kamei, T, Takeyama, H, Sugi, K & Kishimoto, T 2006, 'Infrequent existence of simian virus 40 large T antigen DNA in malignant mesothelioma in Japan', Cancer Science, vol. 97, no. 4, pp. 292-295. https://doi.org/10.1111/j.1349-7006.2006.00171.x
Aoe, Keisuke ; Hiraki, Akio ; Murakami, Tomoyuki ; Toyooka, Shinichi ; Shivapurkar, Narayan ; Gazdar, Adi F. ; Sueoka, Naoko ; Taguchi, Koji ; Kamei, Toshiaki ; Takeyama, Hiroyasu ; Sugi, Kazuro ; Kishimoto, Takumi. / Infrequent existence of simian virus 40 large T antigen DNA in malignant mesothelioma in Japan. In: Cancer Science. 2006 ; Vol. 97, No. 4. pp. 292-295.
@article{2525aafa4826488ebb2dfbd8946fcf46,
title = "Infrequent existence of simian virus 40 large T antigen DNA in malignant mesothelioma in Japan",
abstract = "Malignant mesothelioma is the most common primary pleural neoplasm. Association of simian virus 40 (SV40) with malignant mesothelioma has been reported, suggesting that SV40 plays an important role in the origin of a subset of these tumors. However, significant geographic variation is present as to how often this association occurs. As no study concerning SV40 in malignant mesothelioma has been reported from Japan, we examined the frequency of SV40 infection in Japanese malignant mesothelioma cases. In pleural malignant mesothelioma tissue from 35 patients in Japan, we sought the presence of SV40 large T antigen DNA using real-time polymerase chain reaction (PCR), as well as expression of the viral protein using immunohistological methods. Real-time PCR demonstrated that two of 35 mesotheliomas contained DNA sequences encoding portions of SV40 large T antigen. None of the 35 malignant mesothelioma specimens showed immunoreactivity for SV40 large T antigen. SV40 infection does not appear to have a major role in the development of malignant mesothelioma in Japan.",
author = "Keisuke Aoe and Akio Hiraki and Tomoyuki Murakami and Shinichi Toyooka and Narayan Shivapurkar and Gazdar, {Adi F.} and Naoko Sueoka and Koji Taguchi and Toshiaki Kamei and Hiroyasu Takeyama and Kazuro Sugi and Takumi Kishimoto",
year = "2006",
month = "4",
doi = "10.1111/j.1349-7006.2006.00171.x",
language = "English",
volume = "97",
pages = "292--295",
journal = "Cancer Science",
issn = "1347-9032",
publisher = "Wiley-Blackwell",
number = "4",

}

TY - JOUR

T1 - Infrequent existence of simian virus 40 large T antigen DNA in malignant mesothelioma in Japan

AU - Aoe, Keisuke

AU - Hiraki, Akio

AU - Murakami, Tomoyuki

AU - Toyooka, Shinichi

AU - Shivapurkar, Narayan

AU - Gazdar, Adi F.

AU - Sueoka, Naoko

AU - Taguchi, Koji

AU - Kamei, Toshiaki

AU - Takeyama, Hiroyasu

AU - Sugi, Kazuro

AU - Kishimoto, Takumi

PY - 2006/4

Y1 - 2006/4

N2 - Malignant mesothelioma is the most common primary pleural neoplasm. Association of simian virus 40 (SV40) with malignant mesothelioma has been reported, suggesting that SV40 plays an important role in the origin of a subset of these tumors. However, significant geographic variation is present as to how often this association occurs. As no study concerning SV40 in malignant mesothelioma has been reported from Japan, we examined the frequency of SV40 infection in Japanese malignant mesothelioma cases. In pleural malignant mesothelioma tissue from 35 patients in Japan, we sought the presence of SV40 large T antigen DNA using real-time polymerase chain reaction (PCR), as well as expression of the viral protein using immunohistological methods. Real-time PCR demonstrated that two of 35 mesotheliomas contained DNA sequences encoding portions of SV40 large T antigen. None of the 35 malignant mesothelioma specimens showed immunoreactivity for SV40 large T antigen. SV40 infection does not appear to have a major role in the development of malignant mesothelioma in Japan.

AB - Malignant mesothelioma is the most common primary pleural neoplasm. Association of simian virus 40 (SV40) with malignant mesothelioma has been reported, suggesting that SV40 plays an important role in the origin of a subset of these tumors. However, significant geographic variation is present as to how often this association occurs. As no study concerning SV40 in malignant mesothelioma has been reported from Japan, we examined the frequency of SV40 infection in Japanese malignant mesothelioma cases. In pleural malignant mesothelioma tissue from 35 patients in Japan, we sought the presence of SV40 large T antigen DNA using real-time polymerase chain reaction (PCR), as well as expression of the viral protein using immunohistological methods. Real-time PCR demonstrated that two of 35 mesotheliomas contained DNA sequences encoding portions of SV40 large T antigen. None of the 35 malignant mesothelioma specimens showed immunoreactivity for SV40 large T antigen. SV40 infection does not appear to have a major role in the development of malignant mesothelioma in Japan.

UR - http://www.scopus.com/inward/record.url?scp=33645341728&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645341728&partnerID=8YFLogxK

U2 - 10.1111/j.1349-7006.2006.00171.x

DO - 10.1111/j.1349-7006.2006.00171.x

M3 - Article

C2 - 16630121

AN - SCOPUS:33645341728

VL - 97

SP - 292

EP - 295

JO - Cancer Science

JF - Cancer Science

SN - 1347-9032

IS - 4

ER -