Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana

Tomonori Deguchi, Mariko Itoh, Hiroko Urawa, Tomohiro Matsumoto, Sohei Nakayama, Takashi Kawasaki, Takeshi Kitano, Shoji Oda, Hiroshi Mitani, Taku Takahashi, Takeshi Todo, Junichi Sato, Kiyotaka Okada, Kohei Hatta, Shunsuke Yuba, Yasuhiro Kamei

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.

Original languageEnglish
Pages (from-to)769-775
Number of pages7
JournalDevelopment Growth and Differentiation
Volume51
Issue number9
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Oryzias
Zebrafish
Arabidopsis
Lasers
Genes
Gene Expression
Fishes
Caenorhabditis elegans
Reporter Genes
Shock
Hot Temperature
Research

Keywords

  • Arabidopsis
  • Gene expression
  • Heat shock
  • Medaka
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

Cite this

Deguchi, T., Itoh, M., Urawa, H., Matsumoto, T., Nakayama, S., Kawasaki, T., ... Kamei, Y. (2009). Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana. Development Growth and Differentiation, 51(9), 769-775. https://doi.org/10.1111/j.1440-169X.2009.01135.x

Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana. / Deguchi, Tomonori; Itoh, Mariko; Urawa, Hiroko; Matsumoto, Tomohiro; Nakayama, Sohei; Kawasaki, Takashi; Kitano, Takeshi; Oda, Shoji; Mitani, Hiroshi; Takahashi, Taku; Todo, Takeshi; Sato, Junichi; Okada, Kiyotaka; Hatta, Kohei; Yuba, Shunsuke; Kamei, Yasuhiro.

In: Development Growth and Differentiation, Vol. 51, No. 9, 12.2009, p. 769-775.

Research output: Contribution to journalArticle

Deguchi, T, Itoh, M, Urawa, H, Matsumoto, T, Nakayama, S, Kawasaki, T, Kitano, T, Oda, S, Mitani, H, Takahashi, T, Todo, T, Sato, J, Okada, K, Hatta, K, Yuba, S & Kamei, Y 2009, 'Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana', Development Growth and Differentiation, vol. 51, no. 9, pp. 769-775. https://doi.org/10.1111/j.1440-169X.2009.01135.x
Deguchi, Tomonori ; Itoh, Mariko ; Urawa, Hiroko ; Matsumoto, Tomohiro ; Nakayama, Sohei ; Kawasaki, Takashi ; Kitano, Takeshi ; Oda, Shoji ; Mitani, Hiroshi ; Takahashi, Taku ; Todo, Takeshi ; Sato, Junichi ; Okada, Kiyotaka ; Hatta, Kohei ; Yuba, Shunsuke ; Kamei, Yasuhiro. / Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana. In: Development Growth and Differentiation. 2009 ; Vol. 51, No. 9. pp. 769-775.
@article{d24f22c85eed4d48a285a4916fc2698f,
title = "Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana",
abstract = "Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.",
keywords = "Arabidopsis, Gene expression, Heat shock, Medaka, Zebrafish",
author = "Tomonori Deguchi and Mariko Itoh and Hiroko Urawa and Tomohiro Matsumoto and Sohei Nakayama and Takashi Kawasaki and Takeshi Kitano and Shoji Oda and Hiroshi Mitani and Taku Takahashi and Takeshi Todo and Junichi Sato and Kiyotaka Okada and Kohei Hatta and Shunsuke Yuba and Yasuhiro Kamei",
year = "2009",
month = "12",
doi = "10.1111/j.1440-169X.2009.01135.x",
language = "English",
volume = "51",
pages = "769--775",
journal = "Development Growth and Differentiation",
issn = "0012-1592",
publisher = "Wiley-Blackwell",
number = "9",

}

TY - JOUR

T1 - Infrared laser-mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana

AU - Deguchi, Tomonori

AU - Itoh, Mariko

AU - Urawa, Hiroko

AU - Matsumoto, Tomohiro

AU - Nakayama, Sohei

AU - Kawasaki, Takashi

AU - Kitano, Takeshi

AU - Oda, Shoji

AU - Mitani, Hiroshi

AU - Takahashi, Taku

AU - Todo, Takeshi

AU - Sato, Junichi

AU - Okada, Kiyotaka

AU - Hatta, Kohei

AU - Yuba, Shunsuke

AU - Kamei, Yasuhiro

PY - 2009/12

Y1 - 2009/12

N2 - Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.

AB - Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo, single-cell gene induction using an infrared laser-evoked gene operator (IR-LEGO) system in living nematodes (Caenorhabditis elegans). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR-LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes) and zebrafish (Danio rerio), and a higher plant (Arabidopsis thaliana). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR-LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.

KW - Arabidopsis

KW - Gene expression

KW - Heat shock

KW - Medaka

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=70849103058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70849103058&partnerID=8YFLogxK

U2 - 10.1111/j.1440-169X.2009.01135.x

DO - 10.1111/j.1440-169X.2009.01135.x

M3 - Article

C2 - 19843153

AN - SCOPUS:70849103058

VL - 51

SP - 769

EP - 775

JO - Development Growth and Differentiation

JF - Development Growth and Differentiation

SN - 0012-1592

IS - 9

ER -