Influences of everninomicin, vancomycin and teicoplanin on chemical mediator release from rat peritoneal mast cells

Y. Sugimoto, Y. Iba, K. Utsugi, C. Kamei

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We examined influences of certain antibiotics on the release of chemical mediators from isolated rat peritoneal mast cells in vitro. Isolated peritoneal mast cells were obtained from male Wistar strain rats. Everninomicin (0.06 - 1.2 mg/ml), vancomycin (0.05 - 1.0 mg/ml), teicoplanin (0.07 - 1.4 mg/ml) and concanavalin A (0.01 mg/ml) were used. Isolated mast cells were incubated in the presence of various concentrations of the test compound at 37°C for 10 min. Histamine contents of the supernatant and cell pellet were measured by an automated fluorometric method. Prostaglandin D2 (PGD2) contents of the supernatant were determined by enzyme immunoassay. Everninomicin (0.06 - 1.2 mg/ml) had no influence on histamine and PGD2 release from mast cells. On the other hand, vancomycin significantly released both histamine (0.5 and 1.0 mg/ml) and PGD2 (1.0 mg/ml) from mast cells, but vancomycin did not affect concanavalin A-induced histamine and PGD2 release. Teicoplanin (more than 0.07 mg/ml) significantly stimulated histamine and PGD2 release from mast cells and it also significantly potentiated concanavalin A-induced histamine and PGD2 release. These results suggest that everninomicin causes no chemical mediator release from mast cells, different from vancomycin and teicoplanin.

Original languageEnglish
Pages (from-to)300-305
Number of pages6
JournalJapanese Journal of Pharmacology
Volume83
Issue number4
DOIs
Publication statusPublished - Sep 23 2000

Keywords

  • Everninomicin
  • Histamine release
  • Prostaglandin D release
  • Teicoplanin
  • Vancomycin

ASJC Scopus subject areas

  • Pharmacology

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