Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells

Andrea P. Rodriguez, Hidetsugu Tsujigiwa, Mehmet Gunduz, Beyhan Cengiz, Noriyuki Nagai, Ryo Tamamura, Silvia S. Borkosky, Tohru Takagi, Miho Inoue, Hitoshi Nagatsuka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/Al cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/Al cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.

Original languageEnglish
Pages (from-to)39-47
Number of pages9
JournalBiocell
Volume33
Issue number1
Publication statusPublished - 2009

Fingerprint

Gene Expression
Proteins
Odontoblasts
Dentin
Cell Line
Dental Papilla
Bone and Bones
Dental Pulp
SCID Mice
Tissue Engineering
Osteoblasts
Mesenchymal Stromal Cells
Osteogenesis
Tooth
Theoretical Models
Stem Cells
Cell Culture Techniques
dentin sialophosphoprotein
In Vitro Techniques

Keywords

  • Diffusion chamber
  • Immunohistochemical analysis
  • KUSA/Al cells
  • MDPC-23 cells
  • RT-PCR analysis

ASJC Scopus subject areas

  • Cell Biology

Cite this

Rodriguez, A. P., Tsujigiwa, H., Gunduz, M., Cengiz, B., Nagai, N., Tamamura, R., ... Nagatsuka, H. (2009). Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells. Biocell, 33(1), 39-47.

Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells. / Rodriguez, Andrea P.; Tsujigiwa, Hidetsugu; Gunduz, Mehmet; Cengiz, Beyhan; Nagai, Noriyuki; Tamamura, Ryo; Borkosky, Silvia S.; Takagi, Tohru; Inoue, Miho; Nagatsuka, Hitoshi.

In: Biocell, Vol. 33, No. 1, 2009, p. 39-47.

Research output: Contribution to journalArticle

Rodriguez, AP, Tsujigiwa, H, Gunduz, M, Cengiz, B, Nagai, N, Tamamura, R, Borkosky, SS, Takagi, T, Inoue, M & Nagatsuka, H 2009, 'Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells', Biocell, vol. 33, no. 1, pp. 39-47.
Rodriguez AP, Tsujigiwa H, Gunduz M, Cengiz B, Nagai N, Tamamura R et al. Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells. Biocell. 2009;33(1):39-47.
Rodriguez, Andrea P. ; Tsujigiwa, Hidetsugu ; Gunduz, Mehmet ; Cengiz, Beyhan ; Nagai, Noriyuki ; Tamamura, Ryo ; Borkosky, Silvia S. ; Takagi, Tohru ; Inoue, Miho ; Nagatsuka, Hitoshi. / Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells. In: Biocell. 2009 ; Vol. 33, No. 1. pp. 39-47.
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