Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells

Andrea P. Rodriguez, Hidetsugu Tsujigiwa, Mehmet Gunduz, Beyhan Cengiz, Noriyuki Nagai, Ryo Tamamura, Silvia S. Borkosky, Tohru Takagi, Miho Inoue, Hitoshi Nagatsuka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Progenitor cells play an important biological role in tooth and bone formation, and previous analyses during bone and dentine induction have indicated that they may be a good alternative for tissue engineering. Thus, to clarify the influence of the microenvironment on protein and gene expression, MDPC-23 cells (mouse dental papilla cell line) and KUSA/Al cells (bone marrow stromal cell line) were used, both in vitro cell culture and in intra-abdominal diffusion chambers implanted in 4-week-old male immunodefficient mice (SCID mice). Our results indicate that KUSA/Al cells differentiated into osteoblast-like cells and induced bone tissue inside the chamber, whereas, MDPC-23 showed odontoblast-like characteristics but with a low ability to induce dentin formation. This study shows that MDPC-23 cells are especial cells, which possess morphological and functional characteristics of odontoblast-like cells expressing dentin sialophosphoprotein in vivo. In contrast, dentin sialophosphoprotein gene and protein expression was not detected in both cell lines in vitro. The intra-abdominal diffusion chamber appears as an interesting experimental model for studying phenotypic expression of dental pulp cells in vivo.

Original languageEnglish
Pages (from-to)39-47
Number of pages9
JournalBiocell
Volume33
Issue number1
Publication statusPublished - Jul 1 2009

Keywords

  • Diffusion chamber
  • Immunohistochemical analysis
  • KUSA/Al cells
  • MDPC-23 cells
  • RT-PCR analysis

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Rodriguez, A. P., Tsujigiwa, H., Gunduz, M., Cengiz, B., Nagai, N., Tamamura, R., Borkosky, S. S., Takagi, T., Inoue, M., & Nagatsuka, H. (2009). Influence of the microenvironment on gene and protein expression of odontogenic-like and osteogenic-like cells. Biocell, 33(1), 39-47.