Influence of the axial ligand on the cationic properties of the chlorophyll pair in photosystem II from thermosynechococcus vulcanus

Keisuke Saito, Jian-Ren Shen, Hiroshi Ishikita

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Abstract

Influence of the axial ligand of PD1 chlorophyll (D1-His-198) on the Em of monomer chlorophylls PD1 and PD2, and the PD1 •+/PD2 •+ charge ratio was investigated by theoretical calculations using the PSII crystal structure of Thermosynechococcus vulcanus analyzed at 1.9-Å resolution. It was found that the Em(PD1)/Em(PD2) values and PD1 •+/PD2 •+ ratio remained unchanged upon D1-H198Q mutation. However, Em(P D1) was increased in the D1-H198A mutant, resulting in a more even distribution of the positive charge over PD1/PD2. Introduction of a water molecule as an axial ligand resulted in equal E m values and PD1 •+/PD2 •+ ratios between the mutant and wild-type, thus confirming the presence of the water ligand in the mutant.

Original languageEnglish
Pages (from-to)2634-2640
Number of pages7
JournalBiophysical Journal
Volume102
Issue number11
DOIs
Publication statusPublished - Jun 6 2012

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Photosystem II Protein Complex
Chlorophyll
Ligands
Water
Mutation

ASJC Scopus subject areas

  • Biophysics

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Influence of the axial ligand on the cationic properties of the chlorophyll pair in photosystem II from thermosynechococcus vulcanus. / Saito, Keisuke; Shen, Jian-Ren; Ishikita, Hiroshi.

In: Biophysical Journal, Vol. 102, No. 11, 06.06.2012, p. 2634-2640.

Research output: Contribution to journalArticle

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